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STR Polymorphisms of Henan Population and the Study on Central Plains Han Origin of Chaoshanese

Author: XuLiNa
Tutor: HuShengPing
School: Shantou University
Course: Forensic
Keywords: Population Genetics Short tandem repeats Chaoshan crowd Henan crowd Minnan crowd PCR-STR
CLC: D919
Type: Master's thesis
Year: 2008
Downloads: 65
Quote: 0
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Abstract


Background: The short tandem repeats (short tandem repeat, STR) or microsatellite DNA repeat sequences (microsatellite), a class of more widespread as the core unit of tandem repeat formation from 2 to 6 bp in the human genome has a length state of the DNA sequence. STR widely distributed, number, number of changes and the number of repetitions of the core unit, constitute the genetic polymorphism of STR. Research STR polymorphisms, can reveal the history of human origin, migration, and evolution process from the perspective of molecular biology. The Chaoshan bit too far to the southeast corner of our country, the easternmost Guangdong Province and Fujian Province, adjacent to its geographical level city of Shantou, Chaozhou, Jieyang three. Corresponding ChaoShanRen means living in the region and Han Chinese population own unique dialect and habits. The historical records modern ChaoShanRen from ancient Han nationality. Early on Chaoshan population genetic polymorphism of HLA (Human leucocyte antigen), designed from the perspective of molecular biology to verify the Chaoshan attacked from the assumptions of the Han nationality, the results still can not provide genetic Chaoshan Han nationality origin on certain evidence. Objective: To study the the Henan crowd genetic polymorphism at the DNA level, delve into the Chaoshan Central Plains Han origin. Methods: The study fluorescently labeled PCR and capillary electrophoresis combined technology of 200 healthy people in Henan Han 15 STR loci genetic polymorphism, and of Chaoshan, Henan, Minnan three Han population Penta D, Penta E locus genetic polymorphism study, combined with our research group has published the the Chaoshan crowd STR data and new samples (unpublished data), and the collected published in other parts of the crowd STR data of domestic and international publications, Phylip software different populations genetic distance between building the relationship between the high-resolution phylogenetic tree explore Chaoshan crowd origin of the Han nationality and other groups of people. Result: 15 STR loci in Han population in Henan were detected the 142 allele 451 genotypes, Chaoshan, Henan, Three, Taiwanese Han population Penta D, Penta E loci detected allele and genotype number 27 and 116,28 and 117,26 and 95, respectively. Observed value and the expected value after exact test, in addition to the Henan crowd D3S1358 (P = 0.04) loci, the the other populations locus genotype had no significant difference (p gt; 0.05), in line with the Hardy-Weinberg equilibrium. Chaoshan Han population data clustering analysis of selected STR loci CODIS system from the Minnan Han very close, closer from Henan Han; fusion analysis show the Chaoshan and Minnan two, groups have similar ingredients Han nationality and the composition of the Han nationality accounted for greater than 60%. Dendrogram Chinese population of the North-South divide. Conclusion: 15 STR loci in Han population in Henan combination Henan crowd Forensic individual identification and paternity testing needs to meet; Penta D, Penta E locus is highly polymorphic and can be used as an alternative to the establishment of a DNA database loci; analysis showed that the the Chaoshan and Minnan two, groups with consistent genetic similarity and account for a large Central Plains Han origin ingredients; study also showed that there are genetic differences between north and south China population.

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