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Retinal neovascularization (retinal neovascularization, RNV) age-related macular degeneration (age-related macular degeneration, AMD), diabetic retinopathy and retinal vein occlusion and other retinal diseases result in irreversible vision, severe loss of one of the main reasons. Its pathogenesis is very complex, and so far, no unified argument, many scholars retinal neovascular disease pathogenesis and treatment of many new ideas, such as vascular endothelial growth factor (vascular endothelial cell growth factor, VEGF) role. Anti-VEGF drug Avastin increasing number of clinical applications, and has achieved encouraging results. Avastin was developed by the U.S. biotechnology company Genentech (Genentech), a humanized anti-VEGF recombinant murine monoclonal antibody, its molecular structure by the framework regions of the human IgG1 and mouse monoclonal antibody-antigen binding complementarity determining region, can prevent VEGF and vascular endothelial cell surface receptor, thereby inhibiting endothelial cell mitosis, hinder the formation of new blood vessels. The purpose of this test is to be successfully established the rabbit retinal neovascularization in animal models, observation intravitreal injection of Avastin treatment efficacy of rabbit retinal neovascularization, and provide the basis for clinical treatment. Materials and Methods colored rabbit health test object, the application of coagulation of rabbit binocular the disk both sides set bundle retinal vascular coagulation, and the establishment of a rabbit RNV model. Coagulation 2W, FFA, light microscopic examination and histopathology confirmed retinal neovascularization, select 36 (72) rabbit the retinal neovascularization eye, were randomly divided into a treatment group and a control group. The therapy group intravitreal injection of Avastin 1.25mg/0.05ml, the control group, the vitreous cavity injection BSS 0.05ml. After injection 1W, 2W and 4W each animals were sacrificed 12 enucleated 10 Paraffin sections, electron microscopic examination of two rows. Immunohistochemical examination of VEGF factor and F Ⅷ-Rag protein detection of gray values. Normal eyes (8) for the normal control group. 1 rabbit plate on both sides of binocular retinal vascular coagulation two weeks later, FFA, light microscopic examination and histopathology confirmed retinal neovascularization. The electrocoagulation rabbit eyes 80 excluded shape normal fundus no retinal neovascularization 6, electrocoagulation at scleral perforation 2, retinal neovascularization model of the test a success rate of 90%. A 2.FFA display and fundus color photo: the treatment group medication 1W after fluorescein leakage disappeared 2W and 4W review FFA no neovascularization recurrence fluorescence leakage disappear; the control group 1W, 2W and 4W FFA checks angiography early (early arterial or arterial period) that show the fluorescent leak quickly, the latter part of the formation of the high fluorescence; treatment group medication 1W after fundus color photographs show retinal neovascularization subsided; 2W and 4W observe the fundus, and no recurrence of neovascularization. The control group 1W, 2W and 4W observed fundus neovascularization extend. Immunohistochemistry: pigmented rabbit eyes animal models of retinal neovascularization intravitreal injection of Avastin 1.25mg/0.05ml 1W, 2W and 4W when compared with the control group, the immunohistochemistry test results show: the treatment group VEGF factor the expression of the gradation value and the control group is significantly reduced compared to F VIII-RAg protein expression in gradation value significantly reduced compared with the control group. 4 light and electron microscopy showed: after injection 1W, 2W and 4W when compared with the control group, the treatment group, light microscopy slice breakthrough in the number of capillary endothelial cells of the retinal internal limiting membrane lower than the control group. Histologic and ultrastructural no obvious abnormalities observed after injection. Conclusion 1. Applications electrical condenser electrical method on both sides of the condensate rabbit eye disc set bundle retinal vascular, two weeks after the successful establishment of the model of retinal neovascularization. 2.FFA display and fundus color photos of check can be effectively observed before treatment with Avastin, regression of neovascularization. 3 Histological examination of Avastin in the treatment of retinal neovascularization, optical and electron microscopy did not observe clear evidence of retinal toxicity. 4 anti-VEGF drug Avastin intravitreal injection treatment of experimental RNV, the effect is significant, can be as the clinical retinal neovascularization effective treatment method.
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