Dissertation > Excellent graduate degree dissertation topics show

The Expression of MN1 in Acute Myeloid Leukemia and Its Prognostic Significance in Other Known Genetic Markers

Author: SunHaiMin
Tutor: LiJianYong;QianSiXuan
School: Nanjing Medical University
Course: Internal Medicine
Keywords: Acute myeloid leukemia MN1 FLT3 NPM1 Quantitative PCR Prognostic factors
CLC: R733.7
Type: Master's thesis
Year: 2009
Downloads: 13
Quote: 0
Read: Download Dissertation

Abstract


Purpose . Study MN1 (meningioma 1) gene in acute myeloid leukemia (acute myeloid leukemia, AML) expression correlation to explore MN1 expression with clinical data . 2 . Significance of MN1 gene of known cytogenetic and molecular genetics context . Method 1 by fluorescence quantitative PCR (qRT-PCR) method to detect the expression level of MN1 , including 75 cases of AML and 17 cases of remission (CR) in patients with bone marrow or peripheral blood samples and 11 normal controls . The Mann-Whitney U test to compare different grouping MN1 expression level differences . Analysis MN1 expression with age , sex , blood first visit , CR rate and early death (ED) and multivariate analysis was performed using logistic regression . 2 . Detection of NPM1 and FLT3 mutations . The while detect NPM1 A type and B-type mutations using allele - specific PCR MN1 expression in the context of different cytogenetic and molecular genetics , rate comparison with the χ2 test multiple samples using Kroskal- Wallis H rank sum test . Results 1 . Onset , recurrence and CR group MN1 No difference was found in the expression of dynamic observation , but 8 patients , 4 cases of CR patients MN1 expression did not significantly change compared with before induction , including three cases of recurrence within 5-6 months MN1 expression and the onset of the three patients are higher than the the MN1 median expression levels . MN1 expression was higher early mortality ( P = .016) , logistics regression analysis, age (P = .011) and MN1 expression (P = .004) risk factors for early death . 2 allele-specific PCR simultaneously detect NPM1 A -type and B-type mutations , this method is fast , easy and high sensitivity . Not observed differences MN1 in AML risk stratification ; / relapsed AML patients study of the initial issuance of the 75 cases , compared with MN1 high expression group , MN1 low expression of NPM1 / FLT3- related ( P = .009) . AML study of 64 patients with newly reached similar conclusions ( P = .01) . Conclusion 1. MN1 continuing high levels of expression may be a high recurrence rate ; MN1 expression was higher ED rate (P = .016), age confounding factors affect ED exclude the impact of age , still show high MN1 expression of early risk factor for death . Both in the early rounds / relapsed AML or just the initial issuance of the AML , MN1 low expression of NPM1 / FLT3- related .

Related Dissertations

  1. Gene Cloning and Expression Analysis of Odorant Receptor and Arginine Kinase Gene from Helicoverpa Assulta (Guenée),S433.4
  2. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  3. Oshima Ye Lu Ju CcSOS1 Cloning and Expression Analysis,S682.11
  4. Studies on the Plasma DNA Quantification and the Gene Expression Profiles of Patients with Acute Pancreatitis,R576
  5. Effects of Simvastatin on WT1/hDMP1 Gene Expression Profiles of Human Acute Myeloid Leukemia Cells Lines,R733.71
  6. Investigations on Transcription Level Differences of the DNA Damage-responsive Genes Rad9, Rad1 and Hus1 Among Various Mouse Tissues and Their Transcription Response to Gamma Ray Irradiation,Q691
  7. The Clinical Significance and Correlation Study of AML1-ETO9a Isoform in AML-M2 with T(8;21),R733.7
  8. The Expression and Clinical Significance of CREB in Acute Myeloid Leukemia and Myelodysplastic Syndromes,R551.3
  9. Molecular Cloning, Expression Analysis of Defence Related Genes from Citrus Infected by Ca. Liberibacter Asiaticus,S436.66
  10. Interactions between Artemisinin Drugs and Drug-metabolizing Enzyme,R96
  11. The Expression and Significance of SUMOylation in Insulin-like Growth Factor Ⅰ Receptor in Acute Myeloid Leukemia,R733.71
  12. And aged patients with acute myeloid leukemia treatment summary,R733.71
  13. Toll Like Receptor 2 and 9 Express in Peripheral Blood Monocyte of Chronic Severe Hepatitis B,R512.62
  14. VEGF pretreatment on bone marrow mesenchymal stem cells in the heart rate of retention,R54
  15. Effects of Different Long-term Fertilizations on Community Properties and Functions of Methanotrophs of Black Soil in Northeastern China,S154.3
  16. The Therapeutic Effect of Zishen Blood Capsules in the Treatment of Consolidation Chrmotherapy for Acute Myeloid Leukemia and Its Potential Mechanism,R733.7
  17. To Detect the Expression of NF-kB in Acute Myeloid Leukemia by Real-time Quantitative Polymerase Chain Reaction,R733.7
  18. Study on Cantharidin Intervention in Acute Myeloid Leukemia Telomerase Reverse Transcrptase (hTERT) Methylation,R733.7
  19. The Research on Primary Tupaia Hepatocytes as an Infection Model of Hepatitis C Virus,R735.7
  20. Classical and Molecular Cytogenetic Abnormalities of Adult Acute Myeloid Leukemia,R733.7

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile