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The Expression and Distribution of Aquaporins(aqps) in Gastric Cancer Tissue

Author: ZhuZhengCai
Tutor: WuWenXi
School: Nanjing Medical University
Course: Department of General Surgery
Keywords: Aquaporin Gastric cancer RT-PCR Western-blot Immunohistochemistry
CLC: R735.2
Type: Master's thesis
Year: 2009
Downloads: 13
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Abstract


Objective: To detect aquaporin family (AQPs) in gastric cancer cell lines MKN45, AGS and the SGC7901 cells, normal gastric epithelial cell line GES-1, gastric carcinoma cancer and adjacent normal tissue expression and distribution discussed in gastric cancer Possible role. Method: the principle of informed consent to take tissue samples of the Jiangsu Provincial People's Hospital, Gastrointestinal Surgery resected from June 2007 to September 2008, respectively by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot ( Western-blot), immunohistochemistry (Immunohistochemistry) and immunofluorescence (immunofluorescence) methods to detect AQPs expression and distribution of gastric cancer tissue and cancer adjacent normal tissue, simultaneous detection of gastric cancer cell lines MKN45, AGS, of SGC7901 cells and normal human gastric cell lines GES-1 expression AQPs. Results: AGS, of SGC7901 cells GES-1 cell lines express AQP0, 1,3,4,5,7,11 mRNA; poorly differentiated cell line MKN45 only AQP3 mRNA expression. AQP-0 in human gastric carcinoma and adjacent normal tissue, 2,6,7,8,9,10,12 mRNA were not expressed; gastric carcinoma cancer and adjacent normal tissue the AQP1 and AQP11 mRNA expression of AQP1 AQP11 GAPDH gray value statistical analysis confirmed expression in normal tissue of AQP1 and AQP11 mRNA in gastric carcinoma and paraneoplastic no significant differences (both P gt; 0.05); AQP4mRNA expression in the adjacent normal tissue rich in gastric cancer tissues or expressed at low levels compared with GAPDH gray value confirmed by statistical analysis, AQP4mRNA expression in gastric cancer tissues compared with cancer adjacent normal tissue expression was significantly decreased (P lt; 0.01); of AQP3 in mRNA in gastric cancer tissue and adjacent normal tissue expression of AQP3, and GAPDH gray value compared by statistical analysis confirmed, AQP3 mRNA expression increased significantly (P lt; 0.01) than cancer and adjacent normal tissue; AQP5 mRNA in gastric carcinoma no expression in adjacent normal tissue, the expression in gastric carcinoma, the difference was statistically significant (P lt; 0.01); detected by RT-PCR, AQP3, AQP4, AQP5 mRNA expression in tumor tissue and normal tissue there are obvious differences further by Western-blot detection of AQP3 of AQP4 and AQP5 protein expression level between normal tissue and tumor tissue paraneoplastic AQP3 and AQP5 protein expression results showed that gastric cancer tissue, normal tissue AQP4 protein expression , which further confirms the results of the RT-PCR. Immunohistochemical results showed that AQP4 positive reaction brownish yellow, mainly present in the gastric chief cells and parietal cell membrane, its expression was not detected in gastric carcinoma; immunofluorescence showed of AQP3 AQP5 expression in tumor tissue rich while expression was significantly decreased in the adjacent normal tissue. AQP3 and AQP5 expression with clinical and pathological features of patients with gastric cancer in the gastric tissue analysis, found that poorly differentiated adenocarcinoma AQP3 expression was significantly higher than the well-differentiated adenocarcinoma of AQP3 in and AQP5 expression in gastric cancer patients with lymph node metastasis was positively correlated with gastric cancer patient's age, gender, tumor size, location, and depth of invasion. Conclusion: The results of this study suggest that AQP3, of AQP4 and AQP5 in gastric carcinoma cancer and adjacent normal tissue between differentially expressed; 2. Poorly differentiated adenocarcinoma AQP3 expression was significantly higher than that of well-differentiated adenocarcinoma, AQP3 and AQP5 The expression levels and gastric cancer patients with lymph node metastasis were positively correlated, suggesting that AQPs may play a role in the development of gastric carcinogenesis.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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