Dissertation > Excellent graduate degree dissertation topics show

The Relationship Between the Variation of Leukemia Inhabitory Factor and Endometrium Reception Ability During the Menstrual Cycle

Author: LiYongLi
Tutor: XuXian
School: Ningxia Medical University
Course: Obstetrics and Gynaecology
Keywords: Leukemia inhibitory factor Endometrial receptivity Menstrual cycle Mid-luteal In vitro fertilization - Embryo transfer
CLC: R714.8
Type: Master's thesis
Year: 2010
Downloads: 93
Quote: 0
Read: Download Dissertation

Abstract

Objective Determination of infertile women of childbearing age early follicular phase, the day of ovum, mid-luteal serum and follicular fluid and endometrium leukemia inhibitory factor (LIF) content, explore the the LIF window of implantation endometrial receptivity, clarify the relationship of the LIF in the changes of the menstrual cycle and endometrial receptivity from the multilayer surface, revealing the possible mechanism of LIF in the implantation process, and thus to improve the implantation rate in human assisted reproductive technology and The clinical pregnancy rate provide a theoretical basis. Methods Affiliated Hospital of Ningxia Medical University Center for Reproductive Medicine implementation of in vitro fertilization - embryo transfer (IVF-ET) / follicle cytoplasm sperm injection (ICSI) treatment of 48 cases of infertile women for the first time, the application of real-time fluorescence quantitative polymerase associated reaction (RT-PCR) method for determination of the same menstrual cycle follicles early, luteal interim endometrial LIFmRNA the expression, and collected the same day serum and IVF-ET cycle mining eggs date of serum and follicular fluid application ELISA method Determination of serum and follicular content of the liquid LIF; SPSS11.5 statistical analysis software. Measurement data using t-test, count data x2 test the correlation between each index using Pearson analysis. (1) follicle early, the day of ovum LIF the luteal mid-serum content there is a significant difference (P lt; 0.05), twenty-two comparison mining LIF egg day serum content was significantly higher than the follicle early and luteal medium-term, mid-luteal slightly higher than early follicular phase; (2) follicle the early endometrial LIFmRNA expression amount was significantly higher than the mid-luteal phase (20.70 ± of 12.15vs1.07 ± 1.48, P lt; 0.05); (3) early follicular phase, the day of ovum, mid-luteal serum E2 P there is a significant difference (P lt; 0.001), the twenty-two comparison mining egg day was significantly higher than the follicle early and luteal medium-term, mid-luteal higher than the early follicular phase; (4) luteal interim uterine endometrial LIFmRNA expression amount and serum P content was a significant positive correlation (r = 0.287, P lt; 0.05); (5) the day of ovum serum with the left and right side follicular fluid LIF content was significantly positively correlated (right r = 0.808, the left r = 0.405, P lt ; 0.05); (6) the day of ovum serum and the left and right side follicular fluid LIF content and E2 content showed a significant positive correlation (the the Serum r = 0.990, the right side of r = 0.893, the left r = 0.968, P are lt; 0.05 ); (7) the number of oocytes collected eggs daily serum LIF content and laboratory was a significant positive correlation (r = 0.659, P lt; 0.05); left and right side of LIF in follicular fluid content with ipsilateral number of oocytes was significantly correlated ( the right side of r = 0.540, the left r = 0.431, P are LT; 0.05); the the right follicular fluid of the day of ovum LIF content higher than the left (67.28 for ± 46.15vs54.44 ± 33.09, P = 0.052); (8 ) pregnancy group early follicular phase serum LIF levels significantly lower than non-pregnant group (P lt; 0.05), and the day of ovum, mid-luteal no significant difference between the two groups; (9) pregnancy group the day of ovum serum and follicular fluid of the left and right side of LIF content and serum E2 levels showed a significant positive correlation (serum r = 0.505, the right side of r = 0.497, the left r = 0.572, P are lt; 0.05); (10) female factor group early follicular and mid-luteal endometrial the LIFmRNA expression were significantly lower than the male factor group (early follicular 17.93 for ± 9.36vs30.04 ± 15.95 luteal interim 0.66 ± 0.77vs2.42 ± 2.35, P are lt; 0.05); (11) day of ovum female factor serum LIF content and follicular fluid and serum E2 was significantly positively correlated (serum r = 0.504, the right side of r = 0.471, the left r = 0.420, P are lt; 0.05) Conclusion (1) follicle early the day of ovum, LIF content of the luteal interim serum significant difference prompt LIF source of the site at any time relative to the menstrual cycle varies; follicle early endometrial LIFmRNA the expression was significantly higher than the mid-luteal phase, showed that the uterus The endometrium is the main source; day of ovum serum LIF content was positively correlated with the content of the left and right side of the follicular fluid LIF, serum LIF day of ovum from the ovarian cycle of IVF-ET; (2) early follicular phase, mining egg days, the change trend of the LIF level of the luteal interim serum and serum E2, P content curve consistent, E2, P directly involved in regulating the reproductive cycle of LIF generated; (3) luteal interim endometrial LIFmRNA the expression and serum P content showed a significant positive correlation, indicating that serum P may be raised the endometrium LIFmRNA the endometrial P receptor expression, affect the endometrial embryos; (4) collected eggs daily serum LIF levels and number of oocytes retrieved was significantly positively correlated with serum and follicular fluid in the left and right side LIF content and serum E2 levels also showed a significant positive correlation, LIF content of the left and right side and the left and right side of the follicular fluid was positively correlated with the number of oocytes, the right follicular fluid LIF higher than left, on the day of ovum serum and follicular fluid LIF content is closely related to the number of oocytes retrieved, the right mature ovarian follicles and number of oocytes than the left ovary; (5) Female factors early follicular and mid-luteal endometrial the expression of LIFmRNA were significantly lower than the male factor group, prompt Whatever the causes of female infertility, the endometrium LIFmRNA expression levels diminish, to reduce endometrial receptivity, which is unexplained primary and secondary infertility patients provides the basis for the molecular etiology; (6) Female factor group the day of ovum serum and the left and right side of the follicular fluid LIF content and serum E2 was significantly correlated, suggesting that endometriosis and pelvic tubal factor can affect the growth and maturation of the egg environment; (7) in-depth study of the mechanism of action of LIF in the implantation of the embryo and adjustment factors may provide a theoretical basis for the clinical pregnancy rate and the development of new anti-implantation drugs.

The Relationship Between the Variation of Leukemia Inhabitory Factor and Endometrium Reception Ability During the Menstrual Cycle

Abstract

Related Dissertations