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Study on the Relationship Between Nonalcoholic Fatty Liver Disease in Polycystic Ovary Syndrome and the Polymorphisms of Adiponectin Gene

Author: JingLiHua
Tutor: YiJianPing
School: North China Coal Medical
Course: Obstetrics and Gynaecology
Keywords: Non - alcoholic fatty liver disease Polycystic ovary syndrome Adiponectin Gene polymorphism Chinese Han
CLC: R575.5
Type: Master's thesis
Year: 2010
Downloads: 46
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Abstract


Purpose adiponectin gene (Apm-1) polymorphism in polycystic ovary syndrome (PCOS) and non-alcoholic fatty liver disease (NAFLD) one of the hotspots of genetic predisposition risk. In this study, mainly through the detection adiponectin gene outside exon 45 and 276 of the second intron polymorphism distribution analysis of adiponectin gene polymorphism and the clinical impact factors concurrent with PCOS NAFLD correlation analysis of the relationship between adiponectin gene polymorphism frequency distribution and serum adiponectin levels, explore the adiponectin gene exon 45 and the second includes sub-section 276 polymorphism with PCOS concurrent the NAFLD genetic susceptibility relationship to provide a theoretical basis for the prevention and treatment of PCOS concurrent NAFLD occurred. A case-control study, select the PCOS patients alone 100 cases admitted to the period from March 2008 to September 2009 in Tangshan City, MCH Center for Reproductive infertility clinic as a control group, 100 cases of PCOS concurrent NAFLD patients as case group. Face-to-face way to fill in the questionnaire, including age, waist circumference, hip circumference, height, weight, drinking to collect survey of general information; Determination of biochemical indicators of various provisions automatic biochemical analyzer; serum insulin by radioimmunoassay ; by immune chemiluminescence detection reproductive hormones; enzyme-linked immunosorbent assay (ELISA) determination of serum adiponectin levels; using polymerase chain reaction - restriction fragment length polymorphism method (PCR-RFLP polymerase chain reaction-restriction fragment length polymorphism) detected Apm-45T gt; G, 276G gt; T gene polymorphism. SPSS16.0 statistical analysis software to analyze the data. Measurement data between the two groups using t test, the two groups compared by analysis of variance, count data using x2 test and multivariate unconditional logistic regression analysis to control for other confounding variables. After statistical analysis of the results in the study population the following results: (1) The two groups of subjects genotype distribution consistent with Hardy-weinberg equilibrium, the age of the subjects with BMI constitutes no difference. (2), waist circumference, waist-hip ratio CHOL, TG, LDL, IR index in case group was significantly higher than PCOS concurrent risk factors for NAFLD, serum adiponectin levels and HDL in the case group was significantly lower in the control group, the the PCOS concurrent for protective factors for NAFLD. (3) Apm-45T gt; G polymorphism distribution point three genotypes in the case group / control group: T / T 71% / 72%, G / T 25% / 20% genotype G / G 4% / 8%; two allele frequency distribution in the case group / control group: T 84% / 82%, G-16% / 18%. 45T gt; G polymorphism genotype and allele frequencies of the difference in the two groups was statistically significant (P gt; 0.05). Apm-1276G gt; the T polymorphic loci distribution of the three genotypes in the case group / control group: T / T 8% / 16%, G / T 36% / 51%, G / G type 56% / 33%; two allele frequency distribution in the case group / control group: T 26% / 42% G 74% / 58%. 276G gt; T polymorphism genotype and allele frequencies of the difference in the two groups was statistically significant (P lt; 0.05). (4) Apm-1 276G gt; T genotype frequencies relative risk analysis, genotype T / T and G / T carriers of the risk of suffering from PCOS concurrent NAFLD is 0.387 times the genotype G / G carriers (95% CI : 0.218-0.687, P lt; 0.05). (5) Apm-145T gt; G three genotypes between serum adiponectin levels there is no difference (p gt; 0.05); Apm-276G gt; T polymorphism loci, mutant allele 276T (T / T, G / T genotype) carriers of serum adiponectin water higher than the average non-276T carriers (G / G type) serum adiponectin levels, especially homozygote TT serum adiponectin levels the most significant. (6) Multivariate logistic regression analysis: Apm-1 276T allele, serum adiponectin levels and HDL NAFLD pathogenesis of PCOS concurrent independent protective factors; the IR index TG, LDL, waist-hip ratio, waist circumference is PCOS an independent risk factor for concurrent NAFLD incidence; pathogenesis but have not found Apm-45T gt; G gene polymorphism with polycystic ovary syndrome complicated with non-alcoholic fatty liver disease (P gt; 0.05). The conclusions of this study indicate that the Han population (1) Apm-1 276G gt; T polymorphism with PCOS concurrent NAFLD occurs, the 276T allele carrying mutations occur the PCOS concurrent independent protective factors for NAFLD; not found Apm-1 45T gt; G polymorphism with PCOS concurrent NAFLD incidence. (2) Apm-276G gt; T polymorphisms influence the expression of serum adiponectin high 276T allele carrying a mutation serum adiponectin levels; not found Apm-145T gt; G polymorphisms affect serum adiponectin expression.

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CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease > Liver metabolic disorders
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