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The Effect of Jianxizhuanggu Medication on Apoptosis of Cartilage Cells and Regulation of Gene Expression P53 and Bcl-2 in Rats with Knee Osteoarthritis

Author: WangZhengGuang
Tutor: ZhangJianHua
School: Zhejiang University of Traditional Chinese Medicine
Course: Chinese medicine
Keywords: The Kin knee Zhuanggu party Knee Osteoarthritis Chondrocyte apoptosis P53, Bcl-2 Experimental study
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 64
Quote: 0
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Abstract


Objective To observe and health the knee Zhuanggu square rats osteoarthritis efficacy explore the mechanism of its role in the treatment of osteoarthritis , and provide an experimental basis for its research and development for new drugs of traditional Chinese medicine . Method using a random number table, 60 rats were divided into normal group ( A ) 10 and the operation group 50 , the surgical group manufacturing of knee osteoarthritis model . Modeling method : using a modified Hulth manufacture of rat knee osteoarthritis model operation group randomly digital method divided into model group (B), the the bolus the control group ( C ) , of Kin knee Zhuanggu high doses group (D), the health the knee Zhuanggu Fangzhong dose group (E), the Kin knee Zhuanggu Prescription low dose group (F) 5 . Modeling at 7 days after the administration group, high, medium , low dose group daily orally the Kin knee Zhuanggu Prescription extract dilutions 5ml dose equivalent medicines were 8g, 4g, 2g bolus control group daily to bolus aqueous solution 5ml orally , equivalent medicine the 2g, normal and model groups were given 5ml saline orally . Half of the animals were killed a month and 2 months after treatment , respectively , taking the cartilage of the medial femoral condyle to detect groups chondrocyte apoptosis and its regulatory gene P53 , Bcl - 2 expression in the case . The results of the experiment show that the control group bolus Kin the knee Zhuanggu party high dose group , the Kin knee Zhuanggu prescription dose group , of Kin knee Zhuanggu low dose group were to some extent changes in chondrocyte apoptosis hyperthyroidism trend downward P53 overexpression and increased by inhibition of Bcl-2 protein are better than the model control group , there is a significant difference between the treatment group and the control group ( P lt; 0.05 ) . Conclusion chondrocyte apoptosis is one of the mechanisms leading to the onset of osteoarthritis the , Kin knee Zhuanggu can only transformation the chondrocyte apoptosis hyperthyroidism trend , this effect may be related to the impact of P53, Bcl-2 expression and thus protect the cartilage cells to a certain extent , prevent and delay the development of osteoarthritis .

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
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