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Folium is the object of study, the use of silica gel column chromatography, was separated and purified by means of a polyamide column chromatography, Sephadex LH-20 column chromatography, preparative high performance liquid chromatography and repeated recrystallization its flavone compounds, and Applications chemical properties and mass spectral analysis of its structure identified eight flavonoids were isolated and identified: the different the gorse prime (compound 1), different Plantagenet prime -3 \(Compound 2), iso-vitexin (Compound 3), isobutyl Vitex prime -3 \), different Vitex prime -6 \Among them, the compounds 1, 2, 4, 6, and 8 for the first time obtained from Isatis indigotica separation; compound 5 is a new compound. By UV - visible spectrophotometry, rutin as reference substance, establish of Folium total flavonoids content determination method. The results are the rutin in 11.56 57.60μg · mL -1 range a good linear relationship (r = 0.9998), the average recovery was 100.2% (RSD = 1.4% ). Orthogonal experimental design method, to Daqingye total flavonoids evaluation index, of Folium total flavonoids sample preparation methods to inspect and determine the optimal sample preparation method is 20 times the amount of 60% ethanol, ultrasonic extraction of 4 times, each time 30 min. SOD kit, the eight flavonoids Daqingye total flavonoids extract liquid, remove the ability of the superoxide anion radical was measured to evaluate their antioxidant activity. Results the eight flavonoids Daqingye total flavonoids extract have different levels of antioxidant activity. Simultaneous determination of the Folium 6 flavonoids: different Plantagenet -3 \-O-glucopyranoside different Plantagenet prime, iso-vitexin and iso-orientin glucoside capillary electrophoresis method. different Plantagenet -3-O-glucopyranoside, iso-vitexin - 3-O-glucopyranoside, iso-orientin glucosides -3-O-glucopyranoside, different gorse prime, different vitexin and iso-orientin glycosides concentration 33.0 ~ 660μg · mL -1 , 10.0 ~ 200μg · mL -1 , 14.5 ~ 290μg · mL -1 , 27.5 ~ 550μg · mL of -1 33.0 ~ 660μg · mL -1 and 12.0 ~~ 240 μg · mL -1 range and the peak area was good linear relationship. The recoveries were 97.7% (RSD = 1.8%), 97.5% (RSD = 1.9%), 95.3% (RSD = 3.4%), 91.6% (RSD = 2.0%), 96.3% (RSD = 3.1%) and 98.3% (RSD = 1.8%). The method for Folium quality control to provide a new method.
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