|
Objective: explore health pill to treat idiopathic epilepsy mechanism from the perspective of the brain amino acid neurotransmitter . Methods: 60 SD rats were randomly divided into normal control group , model group , valproate group health pill low, middle, and high dose group ( n = 10 ) . Pentylenetetrazol (PTZ) sub- convulsive dose modeling . Epileptogenic rat epilepsy attack behavior change observed after drug treatment, the morphological changes of hippocampal and cortical pathology , as well as immune staining (SABC) is detected in the hippocampal CA1 , CA3, teeth shaped gyrus (DG) , and parietal cortex (PC), γ-amino butyric acid (GABA), glutamic acid (Glu) expression intensity. Results: 1 . Kang pill can prolong seizure latency time and shorten the the convulsions time , improve behavior abnormal state , compared with the model group , this improvement were significant differences ( P lt; 0.01 ) . Model group appear to neuronal degeneration or necrosis main pathological changes , the health pill degeneration, the percentage of necrotic neurons was significantly lower than the model group ; experiment observed PTZ induced seizures in rat hippocampus and cortex GABA positive the decrease in cell number and optical density , while Glu number of positive cells and the optical density is increased, suggesting PTZ also affects the metabolism of the brain GABA and Glu , confirming the mutual relations of the GABA and Glu and epilepsy . Using Kang pill antiepileptic , hippocampus , cortex GABA positive cells and optical density increases, reducing Glu positive cells and optical density . Conclusion : Kang pill can reduce abnormal behavior induced epileptic rats attack the degree mitigate neuronal damage induced epileptic rats and reduce brain Glu increased GABA levels may be one of the mechanisms play an antiepileptic effect .
|