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Objective: Ecological Medicine from the \Experiments to explore the the different exogenous pathogens factors in normal and immunocompromised mice generally immune status, find the relevant between of exogenous pathogens pathogenic and environmental factors and body condition, laid the theoretical foundation for the modern ecological study of the etiology experimental basis, and further to provide new ideas, new methods for the diagnosis and treatment of exogenous pathogens pathogenic. Methods: 1 Packet: 64 Kunming mice, male and female, weight (20 ± 2) g, were randomly divided into eight groups: normal group, cold group, the cold dampness groups, hot and humid group, low free group cold-free low group, the cold dampness low free group, hot and humid low free group, n = 8. 2 model: (1) the use of the artificial climate chamber to establish a cold, cold, damp heat three kinds of the exogenous pathogens environmental model mice were placed in the the corresponding the exogenous evils environment model, the daily continued to stimulate 8h, seven days in a row. Records mice mental state, activities, hair color, skin, joint, and two will be observed every day, and timing records mice eating and water intake, weight. (2) Preparation of immunocompromised model: mice raised normally two days after each low free model group were given normal mice by intraperitoneal injection of cyclophosphamide (CY) 80mg/kg for three days in a row. Other practice ibid. 3 detection: (1) on the exogenous pathogens in the environment every day, the mice were observed generally change, and record. (2) the cyclophosphamide model placed in the exogenous evils environment for seven consecutive days, drawn. The specific mice eyeball blood, centrifuged, and the serum of mice immune indicators (serum IL-2, IFN-γ content); sterile spleen MTT assay spleen lymphocyte proliferation. Results: 1 as compared with the normal mice, each model mice general changed, showing a series of pathological symptoms. Mouse model activities decreased in the entire modeling period, appear apathetic, the weight growth slowed or weight loss, reduced consumption of drinking water, loose stools, coat lost luster, some mice appear the dorsalis pedis edema and joint swelling large, redness of the skin and so on. Each low-free compared to the model group exogenous pathogens model group, the pathological symptoms earlier and more serious. Impact on the consumption of drinking water, the amount of cold and dampness, hot and humid exogenous pathogens more significant impact on body weight is more significant cold dampness exogenous pathogens. 2 model serum IL-2, IFN-γ content and spleen lymphocyte proliferation was significantly lower than the normal group, there were significant differences (P lt; 0.05 or P lt; 0.01) were statistically. Greater decline in the extent of the various low-free model group. Among the cold dampness exogenous pathogens on serum IL-2 and IFN-γ content and splenic lymphocyte proliferative capacity is relatively more significant. Conclusion: The medical point of view from the ecological body is necessary to \pathogenic species factors. 2 experiments showed pathological state of the body in the long-term \exogenous pathogens of immunocompromised mice its impact is more significant; comprehensive experimental results in the stimulation of cold evil for a long time, the cold dampness evil, damp heat evil, cold dampness evil stimulate immune function is more significant.
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