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Vinegar is rich in nutrients and unique health benefits. Vinegar used in medical history, but for the beauty of functional studies vinegar is less. This study is based vinegar medicinal history, the main research Xiangxi balsamic anti-oxidation, anti-aging, free radicals, eliminate wrinkles, pigmentation and other functions, for the development effectiveness of vinegar health and beauty products provide a theoretical basis. The main results are as follows: using spectrophotometry Xiangxi balsamic Clear Fenton reaction of hydroxyl radical and DPPH radical scavenging ability. Xiangxi balsamic vinegar has a higher hydroxyl radical scavenging rate, clearance rate above 65%. DPPH radical is a stable free radical, at room temperature for 30 min the absorbance of substantially constant. DPPH radical at 1.0 × 10-5 ~ 1.0 × 10-4mol / L concentration range of C and the absorbance A positive linear correlation. Xiangxi balsamic vinegar DPPH half clearance rate (ICso) was 0.293g/mL, controls half vinegar A clearance rate of DPPH (IC50) was 0.229g/mL, vinegar BDPPH half clearance rate control (IC50) was 0.821g/mL. In mice as experimental subjects, by gavage with different doses of Xiangxi balsamic vinegar and control A, control vinegar B30d, determination of serum MDA content, SOD, GSH-Px activity, while setting aged control group and the young control group . Experiments show that vinegar has significant antioxidant effect, compared with aged control group, vinegar serum levels of MDA decreased significantly, SOD, GSH-Px activity was significantly increased, GSH-Px/LPO values ??significantly increased, absolute Most of the group difference was statistically significant (P lt; 0.05), especially in the most significant effect Xiangxi balsamic vinegar (P lt; 0.01). Vinegar vinegar varieties and doses in mice in vivo antioxidant effects have a significant impact, compared to the same dose: high dose group (7.2 mL / Kg), middle dose group (3.6 mL / Kg) and low-dose group (0.6 mL / Kg) are the best in Western vinegar; compared to the same kind of vinegar: vinegar Western each dose group was no significant difference between the control A to the vinegar, the best low-dose, controlled high vinegar B the low-dose group the best. In addition, all three vinegar can significantly reduce body weight in mice (P lt; 0.05). Built using intramuscular progesterone chloasma mouse model fed with different doses of Xiangxi vinegar, vinegar control A, control vinegar B30d, measured mouse liver and skin tissue MDA, NO content, SOD activity, production in mouse skin paraffin, observe the distribution of melanin in the skin. Experiments show that vinegar has significant antioxidant effect, compared with model group, vinegar mice liver and skin tissue MDA content decreased, SOD activity was significantly increased, NO concentration decreased, the vast majority of group differences statistically significant (P lt; 0.05), especially in Western balsamic reduction mouse liver and skin tissue MDA, NO content in skin tissue effect is significant (P lt; 0.01). After feeding vinegar, mouse skin model group was significantly less than the number of melasma, chloasma color depth is also significantly higher than the model group shallow. Vinegar vinegar varieties and doses in mice and fade melasma antioxidant effects have a significant impact, compared to the same dose: high dose group (7.2 mL / Kg) and low-dose group (0.6 mL / Kg ) are the best balsamic vinegar Xiangxi; middle dose group (3.6 mL / Kg) A comparison of vinegar works best. Compared different vinegar: vinegar Xiangxi best low-dose group, controlled dose of vinegar A, the best, the low-dose group B were vinegar works best. On the two-month-old female mice fed different doses of Xiangxi vinegar, vinegar control A, control vinegar B30d, determination of serum and skin tissue MDA content, SOD, GSH-Px activity, production of frozen sections of mouse skin was observed dermal changes in the structure. The results showed a significant Xiangxi balsamic antioxidant effect, compared with the control group, Xiangxi balsamic reduction in serum and skin tissue MDA and GSH-Px increased vitality, enhance the effect of serum SOD activity was significant (P lt ; 0.01). After feeding vinegar, body weight than the control mice had significantly lower (P lt; 0.05), mouse skin texture and delicate, wrinkle reduction than the control group. 7.2 mL / Kg of control vinegar A antioxidant and anti-wrinkle effect is most significant, 7.2 mL / Kg followed by group Xiangxi vinaigrette. This study shows that there is a strong Western vinaigrette with antioxidant activity in vitro in mice is also a strong antioxidant activity, could significantly dilute the vinegar Xiangxi intramuscular progesterone mice melasma and can significantly eliminate the small mouse dorsal skin wrinkles.
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