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Peracetic acid and UV inactivation of enteric viruses

Author: WuJun
Tutor: CaoYuGuang
School: Huazhong University of Science and Technology
Course: Epidemiology and Biostatistics,
Keywords: Poliovirus E. coli bacteriophage f2 Peracetic acid Ultraviolet Disinfection
CLC: R187
Type: Master's thesis
Year: 2009
Downloads: 57
Quote: 0
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Abstract


Poliovirus (PV) representative of enteroviruses, strong resistance to the disinfectant. Biological properties of E. coli bacteriophage f2 with hepatitis A virus, PV similar resistance to disinfectants and pathogenic enterovirus was close. This study attempts to disinfect these two viruses to evaluate disinfectants disinfection. Peracetic acid (PAA) solution against bacteria, fungi etc. have a strong performance inactivation. Ultraviolet (UV) disinfection against bacteria, viruses and other pathogenic microorganisms inactivation effect is stronger, safer drinking water after disinfection. In this study, PV-I of the Sabin strains and E. coli bacteriophage f2 virus as drinking water disinfection instructions, preparation simulated water sample, using peracetic acid and its joint UV disinfection with sodium hypochlorite disinfection effect compared PAA preliminary study combined with UV disinfection for drinking water disinfection to provide reference data for viruses. The first part of the combination on peracetic acid and UV inactivation of poliovirus type I Objective: To study on peracetic acid combined with UV-inactivated polio virus type I the role of disinfection with sodium hypochlorite to compare and evaluate its results. Methods: The proliferation of poliovirus (PV-I) was dope, simulated water sample preparation. Using end point dilution method PV-I infection titer, was chosen through the identification of effective concentration neutralizer neutralizer. Suspension quantitative inactivation experiments simulated water samples disinfected, add neutralizer terminate disinfection, and then measured PV-I infection titers calculated inactivated logarithm. Results: peracetic acid concentration of 3mg / L, UV intensity of 10mws/cm2, the role of 10min, against the logarithm of inactivated poliovirus 1.75 ± 0.08. Peracetic acid concentration of 3mg / L, UV intensity of 19mws/cm2, the role of 10min, polio virus type I needed to achieve the four pairs of numbers above the level of inactivation. The chlorine content of 3mg / L of sodium hypochlorite inactivated polio virus type I is not strong, the role of 10min only reach 0.31 ± 0.19 logarithmic magnitude. Conclusion: peracetic acid and UV combination on inactivation of poliovirus type I stronger than sodium hypochlorite. With increasing concentration of peracetic acid, UV intensity enhancement of the inactivation of poliovirus type I enhanced role. The second part of the peracetic acid combined with UV inactivation of E. coli bacteriophage f2 Objective: To study on peracetic acid combined with UV inactivation of Escherichia coli bacteriophage f2, and sodium hypochlorite disinfection effects were compared to evaluate their effectiveness. Methods: The proliferation of E. coli bacteriophage f2 was dope, simulated water sample preparation. Agar culture method using double-coli bacteriophage f2 concentration. Through effective concentration was chosen for identification neutralizer neutralizer. Suspension quantitative inactivation experiments simulated water samples disinfected, add neutralizer terminate disinfection, and then the concentration of E. coli bacteriophage f2 was measured. Results: peracetic acid concentration of 3mg / L, UV intensity of 10mws/cm2, the role of 10min, the inactivation of E. coli bacteriophage f2 on the value of 0.54 ± 0.13. When the peracetic acid concentration of 15.0mg / L, when the UV intensity of 27.9mws/cm2, f2 phage inactivation value of 1.62 ± 0.84, UV intensity to 27.9 mws/cm2, peracetic acid concentration of 100mg / L at , f2 bacteriophage inactivation can be achieved logarithmic gt; 4. The chlorine content of 3mg / L of sodium hypochlorite on the role of bacteriophage f2 10min only reach 0.25 ± 0.04 logarithmic magnitude. Conclusion: peracetic acid combined with UV inactivation of E. coli bacteriophage f2 stronger than sodium hypochlorite. With increasing concentration of peracetic acid, UV intensity enhancement of the inactivation of E. coli bacteriophage f2 enhanced role.

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CLC: > Medicine, health > Preventive Medicine,Health > Epidemiology and Prevention of Disease > Disinfection
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