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Of Angelica Angelica sinensis (Oliv.,) Diels as the one of Gansu characteristics herbs, has the blood circulation, relieving pain, and other functions. Traditional nursery methods, however, must be chosen habitat severely damaged Angelica producing areas of native vegetation, and long life cycle, low efficiency, vulnerable to a variety of diseases of the lower production, the decline in quality. Therefore, the use of tissue culture techniques to produce angelica seedlings, on the one hand to provide the best for industrialized breeding process, on the other hand is expected to change the traditional breeding methods of ecological damage, suppression Yaonong habitat reclamation use, protect local existing natural vegetation, and improve the quality of angelica seedlings. First, establish a sterile breeding system, then ① Angelica seed germination the resulting seedlings petiole, apical, and leaves as explants, focusing on different exogenous hormone ratio and different culture conditions affect its organ differentiation ; ② using annual Angelica terminal bud as a material, try adventitious buds ways. The results showed that the surface of the explants after sterilization using 50mg / L of antibiotic solution treatment 30min, the effect is most ideal, and the bactericidal effect of penicillin pollution only 4% to 10% stronger than gentamicin. Tissue culture stage, ① the best medium for callus induction H 0.5mg / L 2,4-D, 2,4-D is indispensable; the explants induced effect order of the petiole, root sharp blades;, H medium is more suitable than the B 5 and MS induced callus; browning control callus effective way is to add 0.1% g / LAc and multiple transfers, brown level is significantly reduced. ② callus Subculture Proliferation suitable medium for the MS 0.5mg/L2 ,4-D 1.0mg/LBA or MS 0.05mg/LNAA 0.5mg/LKT. ③ the callus proliferation after transfer to differentiation medium MS 0.02mg/LNAA 0.1mg/LZT, the differentiation rate up to 98%. ④ callus surface the buds grow by proliferation and strong seedlings transferred to rooting medium 1/2MS 1.0mg/LIBA 300mg/LLH 3% of white sugar, two weeks later, with an average per plant rooting 4 rooting rate up to 100%. ⑤ Angelica terminal bud proliferation pathway MS 5.0mg/LBA 1.0 mg / LIAA combination higher proliferation multiples. ⑥ seedling roots to the root growing stout, first the glare Lianmiao 7d, and then opening the lid closed the light Lianmiao 5d finally transferred to the humus vermiculite (1:1) mixed transplanting matrix survival rate up to 80%. Callus induction stage, lighting conditions appropriate to pre-dark culture, late light training, a temperature of 23 ° C; callus proliferation and differentiation, the culture temperature to 22 ~ 25 ℃ appropriate; rooting culture in a temperature of 20 ~ 22 ℃, pre-incubated in the dark late light culture conducive to rooting; whole incubation period 2000 ~ 3000Lx light intensity, illumination time 16h / d.
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