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Purpose of osteoporosis (osteoporosis, OP) in reduced bone mass mainly generalized systemic disorders , a common disease of the elderly , occurring disease. OP resulting in reduced bone mass, fine structure of bone destruction leading to bone fragility and an increased risk of fracture . Defects in recent years, animal bones of osteoporosis , fracture healing many studies , but more concentrated in the limb long bones , less osteoporosis of the jaw bone defects and fracture healing , the present study, the cause of postmenopausal bone osteoporotic model and mandible bone defect model , bone from the serum and urine biochemical markers , the callus bone tissue morphometry , the callus TGF - β 1 immunohistochemical chemical expression to evaluate the the OP mandible bone defect healing process . Female Wistar rats methods April age weight 33g about 80 , were randomly divided into three groups , namely : castration mandibular mandibular defects in the sham-operated group (sham operation mandibular bone defect, referred to as the S group , 26 ) ; defect group the (OVX mandibular bone defect, referred to as group O , 27 ) ; castration of estrogen therapy mandibular defects group ( OVX estrogen mandibular bone defect , referred to as the group E mandibular defects postoperative day 1 starting 1.5mg / kg body weight given nylestriol orally two times a week , as long-term estrogen replacement therapy group , 27 ) . Castration (OVX) and create a model of osteoporosis , castration postoperative 8 weeks osteoporosis model was established ; then establish the model of the left mandibular defects . The mandibular defects preoperative and postoperative One , two, three , four weeks blood were detected in serum estradiol ( E 2 < / sub> ) , Ca , P , alkaline phosphatase ( ALP) and acid phosphatase enzyme (ACP) level . Mandible bone defects after one, two, three , four weeks , animals were killed , were sacrificed after the preparation of demineralized bone chip , HE staining ( tissue morphology observed ) and Masson staining ( pathology graphic analysis to detect the different groups at different times bone callus degree of maturity ) , and TGF -β in 1 immunohistochemical staining ( pathological graphic analysis detected a different time for different groups of callus
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