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The purpose of learning to master oleic acid (oleic acid, OA) intravenous injection in rats with acute lung injury (acute lunginjury, ALI) method of the model ; investigate pulmonary surfactant (pulmonary surfactant, PS) system changes in acute oleic acid -induced lung injury law and its mechanism of beta - adrenergic receptors (beta-adrenergic receptor, β-AR) agonists - hydrochloric acid isoproterenol (Isoprenaline hydrochloride) on the prevention and treatment of OA induced ALI rats and its possible mechanism of action , open up new therapeutic measures to provide a theoretical basis and practical direction for the clinical treatment of ALI . Methods OA intravenous rat model of ALI . 78 SD rats were randomly divided into three groups : saline control group (NS ) , oleic acid induced acute lung injury group (OA group ) , isoproterenol hydrochloride treatment group (IO group ) . Were observed 1 hour, 2 hours and 4 hours after the blood was collected and quickly put to death , and determination of arterial oxygen tension (PaO 2 ), arterial partial pressure of carbon dioxide ( the PaCO 2 ) , pH values ??and serum lung surfactant protein A (surfactant associated protein A, SP-A). Right lung lavage , measured in bronchoalveolar lavage fluid (bronchoalveolar lavage fluid, BALF) total protein (total proteins, TP), total phospholipids ( the total phospholipids TPL ) , phosphatidylcholine (phosphatidylcholine, PC) and SP- A concentration ; measured left upper segment wet / dry weight ratio (wet / dry weight, W / D value) , the left lung middle for membrane β-AR radioligand binding assays , the left lower lobe pathological examination . Results with the NS group , OA group at each time point in BALF TPL, PC / TPL and SP-A significantly reduced ( P lt; 0.01 ) , TP, W / D values ??and serum SP- A was significantly higher ( P lt ; 0.05) , β-AR lung tissue decreased significantly (P lt; 0.01), the pulmonary pathological see ALI manifestations obvious ; OA group at each time point serum SP-A with the same group of BALF SP-A was a negative correlation (r OA1 = -0.914, P lt; 0.05; r OA2 = -0.923, P lt; 0.01; r OA4 = -0.910, P lt; 0.05) . The IO group BALF TPL , the PC / TPL and the SP - A compared with OA group the same time points significantly rose ( P lt ; 0.05 ) , while of TP, W / D values ??and serum SP - A significantly with lower ( P lt ; 0.05 ) , lung organizations β-AR was no significant sex difference ( P gt ; 0.05 ) of lung performance improved markedly .
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