|
[Background] highland environment the hypoxic important physiological and biochemical reactions is one of the generation of various stress proteins, preliminary studies have been found in an earlier study of radical oxygen environment there is a special ingredient in human blood, spectral scanning has a characteristic absorption peak spectral information expression known mountain sickness related substances are not consistent and the difference is larger, its quality, the amount of mountain sickness was closely correlated, γ = 0.93. The same structure of the substance has the same expression of the hydrogen spectrum, such as expression differences prompted structural differences exist in the structure and function. Then the animal experiments confirmed that the special ingredient in the Wistar rat and rabbit blood under anesthesia absorbance level of conscious animals more significant downward trend, found on the body's nervous, endocrine and immune important regulatory role of β- endorphin inhibit the secretion of the components of the plasma. Its essence and humoral anti-hypoxia stress compounds have a similar function, we call it the stress peptide (the hypoxia stress peptide, HSP). And spot assay suitable for the rapid detection of grassroots. HSP discovery is one of the important achievements of the High Altitude Medicine, but the specific molecular structure and specific correlation with altitude sickness is not very clear. With the progress of proteomics research, new research methods in the study of protein structure and function in an increasingly wide range of applications, mass spectrometry (mass spectrometry, MS), nuclear magnetic resonance H spectrum (1H-nuclear magnetic resonance spectroscopy, 1H-NMR ) C spectrum (13C-nuclear magnetic resonance spectroscopy, 13C-NMR) and IR (fourier transform infrared spectrometry, FITR), UV spectrum (Ultravlolet spectrophotometry, UVS) experiment method to get more attention in the analysis of protein structure. This study the above-described method is applied to the structural analysis of the stress peptide. [Objective] pure stress peptide purified using mass spectrometry, NMR H spectra, NMR C spectrum as well as infrared and ultraviolet spectrum of its formula, molecular structure, amino acid composition analysis, and into the highland part anxious crowd plateau patients with pulmonary edema, plateau trauma patients, and the plateau load exercise stress peptide levels detected, explore its correlation with altitude sickness. [] 1, take the 29 cases of acute high altitude illness and hypoxic rabbit model serum by high-speed centrifugation, ion exchange chromatography
|