|
Objective: To establish animal models of diabetes in rats , observed the model group and the statin drug group , the rat neuromuscular morphological changes as well as the muscle fibers within the enzyme optical density of gray value changes of diabetic myopathy mechanism of statins on diabetic skeletal muscle lesions . Method : weighing 200 ~ 240g SD male rats 40 were randomly divided into four groups of 10 each , divided into a control medication group (A ) , normal control group (D ) , diabetic model group (C cutting ) , the model medication group (B ) with streptozotocin diabetic model made ??of group B and group C , A, B rats fluorine statin gavage , C , D group with the same amount of tap water gavage . In six weeks , eight weeks from rat quadriceps muscle and the sural nerve , the nerve under a dissecting microscope isolated single nerve fiber light microscope myelin and axonal change , take the 1 × 1 × 1 cm two part of the muscle tissue , part of the rapidly cooled by the liquid nitrogen , in the frozen section machine serial sectioning line enzyme histochemistry , to observe muscle fiber morphology , genotyping , enzyme changes , and the resulting image input cells medical image analysis system lesions the size of the area , the gray value and the optical density values ??, part of the ultrastructural changes made ??semi - thin sections observed by transmission electron microscopy . Results: Rats in the monitoring of blood glucose , take the tail vein injection of streptozotocin standard animal model of blood sugar reaches into the mold after 72 hours , group B , C rats 8 weeks of blood glucose has been maintained at a high the level of group B blood sugar value is lower than the C group and P lt ; 0.05 between the two , there were statistically significant , blood cholesterol (TC), serum triglyceride (TG) 6 weeks , 8 weeks two points in time comparable group C gt; B group gt ; groups D , B , C group comparison P lt; 0.05 statistical significance. Group B blood cholesterol
|