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Objective To study YIGUTANG treatment of osteoporosis in ovariectomized rats efficacy , and to explore its mechanism . Methods 12 -week-old female wistar rats were 40 , of which 30 in the Ketamine (5mg/100g) under anesthesia , open the abdominal cavity to remove both ovaries , sutured , and the other 10 are not to be simply open the abdominal cavity ovariectomized . The rats were divided into group A ( model group ), B group ( treatment group ), C group ( positive control group ), D group ( sham group ) four groups after 12 weeks , were to be saline (10ml / kg), YIGUTANG decoction (1g/kg), α-D3 solution (0.05gg/kg) and saline (10ml/kg) gavage . Gavage for 12 weeks , all rats were eyeball blood serum was separated , put stored at -20 ℃ , by radioimmunoassay in serum osteocalcin (BGP), calcitonin (CT); to Electrode Method Ca , P content changes . After blood animals in each group were killed , take the right tibia biopsy done by HE staining on osteoblast cells were observed compared with immunohistochemical staining of BMP-2. By dual-energy X-ray absorptiometry was determined by measuring the right femur metaphyseal bone density. Three-point bending test , record the load -deformation curve , left femur was measured biomechanical indicators . The experimental determination of statistical indicators , analyze, summarize the results. Results After 12 weeks of treatment YIGUTANG decoction orally , significantly increased bone mineral density in ovariectomized rats , serum hormone levels , increasing the number of osteoblasts (P lt; 0.01 or p lt; 0.05), and can improve treated rats bone mechanical properties, but also by immunohistochemical staining of BMP-2 found deep brown staining, significantly higher than the model group . Conclusion YIGUTANG on osteoporosis in ovariectomized rats has a therapeutic effect , can improve
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