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4 - hydroxy isoleucine, leucine insulin secretion activity can existing separation costs are higher as a drug for the treatment of type Ⅱ diabetes is not suitable for industrial production . Therefore, we fenugreek seeds , 4 - hydroxy isoleucine separation methods were studied. According to the 4 - hydroxy isoleucine easily lactones, does not occur epimers isomerization features converted to lactone, the use of 4 - hydroxy isoleucine lactone with the nature of other amino acids in 1M HCl ( aq ) difference, column chromatography on silica gel (chloroform / methanol ) or acetone as the solvent was obtained by solid - liquid extraction separation of lactone and ( 2S , 3R, 4S ) -4 - hydroxy isobutyric leucine lactone in acetone preferential crystallization. Lactone can be hydrolyzed under basic conditions (NaOH) to a 4 - hydroxy- isoleucine and the configuration does not change , thus a large number of easily and to obtain a high purity of the ( 2S , 3R, 4S ) -4 - hydroxy isobutyric leucine . Recent 4 - hydroxy isoleucine research progress , including the form of existence , activity , extraction and separation methods and stereoisomers body synthesis method .
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