Dissertation > Excellent graduate degree dissertation topics show

Niacin on peripheral blood mononuclear cells of cAMP and the role of ABCA1 expression

Author: LiYingQing
Tutor: WenHongYan
School: Nanhua University
Course: Internal Medicine
Keywords: Niacin Monocytes ABCA1 cAMP
CLC: R541.4
Type: Master's thesis
Year: 2006
Downloads: 83
Quote: 0
Read: Download Dissertation

Abstract


Objective: To observe niacin and PKA inhibitor Ro 31-8220 alone and in combination on cultured monocytes ABCA1 expression of cAMP and explore niacin regulate ABCA1 expression and cAMP / PKA pathway correlation. Methods: Patients by coronary angiography and blood lipid examination is divided into three groups: 1. Non-coronary heart disease group (set to blank control group); 2. Coronary heart disease high cholesterol group; 3. CHD normal human blood group; wherein 2,3, respectively, with the blank control group were, niacin, PKA inhibitors, niacin PKA inhibitor four subgroups. Sterile blood samples were collected patients femoral artery, isolated peripheral blood mononuclear cells to cultured cells of each group were incubated for 3 to 48 hours. Select 3,12,24 and 48 hours, etc. 4 time points, the use of reverse transcriptase polymerase chain reaction and Western blot blot were used to detect ABCA1mRNA and ABCA1 protein expression, using low pH was measured by EIA intracellular cAMP content , and explore niacin on monocyte expression of ABCA1 with cAMP correlation. Results: ① The peripheral blood mononuclear cells of ABCA1 expression and caMP content in 3h ~ 48h after this time period no significant change (P> 0.05); ② group of patients with coronary heart disease high blood mononuclear cells of ABCA1 expression of coronary heart disease than non-group monocytes ABCA1 expression was significantly decreased (P <0.05), while the normal coronary blood mononuclear cells of group expression of ABCA1 coronary heart disease than non-group monocytes ABCA1 expression was not significantly difference (P> 0.05); ③ niacin alone can significantly increase coronary heart disease and coronary heart disease high blood groups of normal human blood monocytes group of ABCA1 expression (P <0.05), while both sets of monocytes cAMP content increased (P <0.05); ④ PKA inhibitor Ro 31-8220 alone can significantly reduce coronary heart disease and coronary heart disease high blood group blood group of normal human monocytes ABCA1 expression (P <0.05), while these two sets of cAMP in monocytes decreased (P <0.05); ⑤ nicotinic acid when combined with the PKA inhibitor, niacin cause coronary heart disease and coronary heart disease high blood groups of normal human blood group ABCA1 expression in monocytes upward and the role of increased levels of cAMP was completely inhibited (P <0.05). Conclusion: ① niacin and Ro 31-8220 on peripheral blood mononuclear cells of ABCA1 expression and cAMP content, no clear correlation with time; niacin drug stability; ② peripheral blood mononuclear cells in ABCA1 expression and crown plasma cholesterol levels in patients with heart disease were negatively correlated; ③ niacin can be cAMP / PKA pathway in peripheral blood mononuclear cells increased ABCA1 expression, so that patients with coronary heart disease increased HDL-C levels.

Related Dissertations

  1. The Effect of Tanshinone ⅡA and Niacin on the Cardiovascular Function of Infant Obese Rat,R285.5
  2. Study on Human Peripheral Blood Stem Cells Differentiate into Liver Cells,R575.1
  3. Police Detention Grassroots Work to Explore the Issue of Burnout,D669.2
  4. The issue of prisoners of war in the American Civil War,K152
  5. Expression and Significance of Mst3b after Spinal Cord Injury in Adult Rats,R651.2
  6. The Measure of Rho-kinase of Serum and Peripheral Blood Monocytes in Patients with PAH Realated COPD,R563.9
  7. The cAMP, cAMP/cAMP-related Validation Studies on the Repeated Colds Caused By "Lung-Yang Defieieney" With"Yang-warmed Xin-Gan" Method,R256.1
  8. Construction and Study of ABCA1 Mutants with Arsenic-fast,Q78
  9. Select Breeding and Structure Optimization of Syntrppuic Acetogens Coculture B6,X172
  10. cAMP-dependent protein kinase Fyn agonism on the spinal cord and its relationship formed with chronic inflammatory pain,R96
  11. Osteoblasts actin on cAMP regulation of L-type calcium channels and its molecular mechanism,R966
  12. Research on Co-effect of Insulin and Oligomeric Amyloid Beta on Phosphorylated CAMP Response Element Binding Protein,R749.16
  13. Effects of Anti-fetal Immunity on the Pathogenesis of Hypertensive Disorder Complicating Pregnancy,R714.246
  14. Vascular endothelial growth factor stenosis after balloon injury in rats,R654.3
  15. The Ectopic Expression of LTBR on the SLE’s Peripheral Monocyte and It’s Mechanism Explore,R593.241
  16. The Influence of the Combination of Niacin and Statins on the Vulnerability of Experimental Atherosclerotic,R543
  17. Regulation of Phenotype by Dibutyryl-cAMP in Irradiation-stimulated Murine BV2 Microglial Cells,R329
  18. A Study on the Protection and Development of Chinese Traditional Music from Qu Jiaying’s Concert in Gu’an,J609.2
  19. Humanistic Tendencies of the Hunan TV’s Entertainment Programs,G222
  20. Huizhou Smart Grid Integration with the User Side of Business,TM76
  21. Role of PKA in NMDA Receptor-induced NMDA Receptor Synaptic Accumulation in Spinal Dorsal Horn during Inflammatory Pain,R96

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Coronary arteries ( atherosclerosis ),heart disease (CHD)
© 2012 www.DissertationTopic.Net  Mobile