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Javanica is Simaroubaceae javanica fruit plants, mainly produced in Guangdong, Guangxi and other places. Is a traditional Chinese medicine, with detoxification, cut malaria, dysentery, corrosion wart and other effects. Back in ancient times Chinese folk widely Brucea treatment of amoebic dysentery and other diseases. In recent years, as an adjunct crow gall SEEDS antitumor drugs, causing widespread concern. This paper focuses on javanica oil extraction, separation, trial javanica oil emulsion samples and in vitro anti-tumor effects were studied, main results are as follows: the use of traditional solvent and supercritical CO 2 javanica oil extraction method for extraction, solvent extraction javanica oil optimum conditions as follows: extraction temperature 90 ℃, extraction time of 3 hours, the solid-liquid ratio 1:5, javanica oil extraction rate of 20.2%. Supercritical CO 2 javanica oil extraction the optimum conditions: operating pressure 30Mpa, operating temperature 60 ℃, operating time 100 minutes; javanica oil extraction rate of 19.02%. Analyzed by gas chromatography using two different methods of extraction javanica oil fatty acid composition, the type of extraction method was found to javanica oil fatty acid composition is not affected. Javanica oil fatty acids including palmitic acid 10.12%, 7.39% stearic acid, oleic acid, 60.50%, 20.08% linoleic acid, another javanica oil also contains small amounts of arachidonic acid and wax acids. By gas chromatography - mass spectrometry analysis of the chemical composition javanica oil were detected out of the 30 compounds, in addition to fatty acids, in an organic solvent extraction javanica oil also detected in some of the hydrocarbons. Using conventional methods javanica oil refining, including degumming, deacidification, dehydration, bleaching and other processes. Obtained by conventional solvent extraction javanica oil darker color, contains more pigment, the paper examines the types of bleaching agents, bleaching dosage, temperature bleaching, bleaching time javanica oil bleaching effect. Lovibond assay results showed that the effect of oil bleaching effect on the decolorization links sorted by size followed by bleaching temperature, bleaching time, type of bleaching agent, bleaching agent dosage. Determined by orthogonal experiments javanica oil decolorization optimal conditions for bleaching temperature 100 ℃, bleaching time of 30 minutes, SF-NO-based bleaching agents, bleaching dosage 1%. This paper also studied the different preparation methods resulting javanica oil emulsion stability of the sample were selected Tween80 two kinds of soy lecithin and different hydrophilic emulsifier, study its javanica oil emulsion samples emulsifying effect. The results showed that soybean lecithin as emulsifier, javanica oil: glycerol: water 4:1:35 when javanica oil emulsion sample in a water bath at 60 ℃ can remain stable for 1 hour at room temperature is stable for one month . Investigated by in vitro test tube experiments javanica oil emulsion samples of HL-60 human leukemia, Bel-7402 human hepatoma, BGC-823 human gastric cancer and KB human nasopharyngeal tumor strains inhibitory effect. And the effects of skim crow gall
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