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Study of Mutations in the D-LOOP Region of Mitochondrial DNA in Acute Leukaemia

Author: JiangYuJie
Tutor: CongYaQin
School: Shandong University
Course: Internal Medicine
Keywords: Acute leukemia Mitochondrial DNA D-LOOP area Mutation
CLC: R733.71
Type: Master's thesis
Year: 2005
Downloads: 56
Quote: 0
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Abstract


Study Objective mitochondria (mitochondria) is animal cells In addition to the nucleus only DNA containing cell device , and can not rely nuclear genome replication , transcription and translation , are performed oxidation phosphorylation reaction (oxidative phosphorylation OXPHOS) and for cell provides the energy of the main venues. D-loop region (D-LOOP area ) is the main non - coding region of the mitochondrial DNA (mtDNA) , accounting for about 6% of all mtDNA position 16028bp ~ 577bp (tRNApro and tRNAphe ) , rich in A-T base genetically hypervariable region , replication origin of the mtDNA the heavy and light chain are located in this region , the regulation of mtDNA transcription and replication , studies show that the D-ring is a mutation rate of approximately 10 times in the entire sequence . Highly polymorphic D-loop region the highly polymorphic reflect mitochondrial mutation have been a lot of research work confirmed the area mtDNA mutation hotspot region . In recent years , the research focus of the medical profession , especially in tumor development , for the study of human mtDNA mutations , but reported for acute leukemia patients with mtDNA D-LOOP mutations have not been found . The main purpose of this study is the use of PCR technology and T vector cloning technology extracted and amplified fragment of full-length mtDNA D-LOOP area of bone marrow mononuclear cells in patients with acute leukemia , acute leukemia patients with mtDNA D-LOOP region DNA was measured by direct sequencing method sequence , to explore whether the mutation and mutation type . And summarizes the mutations in patients with acute leukemia D-LOOP area law , to explore the possible mechanism of its occurrence and consequences to provide theoretical reference for molecular biology , pathogenesis , diagnosis and treatment of acute leukemia . Methods 20 patients with acute leukemia by EDTA anticoagulated bone marrow fluid , first isolated using differential centrifugation in lymphocyte separation medium bone marrow mononuclear cells , genomic DNA was extracted with phenol - chloroform extraction method , designed to amplify the full-length primers mitochondrial DNA D-LOOP region upstream primer :

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
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