|
Skeletal muscle apoptosis and movement, the movement of the load and skeletal muscle subcellular structures. Libera, etc. also what type of muscle fiber is easier to study problems apoptosis them less than the fast-twitch that slow muscle apoptosis tendency. Zhou Jie et al's findings show that the slow twitch fibers than fast-twitch muscle fibers more prone to apoptosis. In this study, using different intensity treadmill exercise model, the rat gastrocnemius medial head of research for the study, the relationship between exercise-induced apoptosis in skeletal muscle and muscle fiber type. Methods: 30 adult male SD rats were randomly divided into sedentary control (C, n = 10), moderate-intensity exercise group (PME, n = 10) and high-intensity exercise group (the SHE, n = 10). PME group Treadmill speed 18m · min -1 (equivalent to 60-70% Vo 2max ), sports the 100min, HE group treadmill speed to 28m · min -1 (equivalent to 90% Vo 2max ), sports 20min. Quiet group and the exercise group rats were decapitated and immediately after the quiet state and movement take hindlimb medial head of gastrocnemius, respectively, tested in situ end labeling (TUNEL) apoptosis, the identification of the ATP enzyme staining type Ⅰ and type II skeletal muscle fibers, SDS-PAGE gel electrophoretic separation of MHC Ⅰ, MHC Ⅱ a, MHC Ⅱ b and MHC Ⅱ x. Results: (1) in quiet control rats gastrocnemius medial head detected a small amount of TUNEL positive stained nucleus; moderate-intensity exercise group scattered TUNEL positive stained nucleus, apoptotic index was 19.60 ± 6.18%; high intensity exercise The group also detect TUNEL positive stained nucleus, apoptotic index was 11.70 ± 4.61%. (2) moderate-intensity exercise group (PME), apoptosis index and the percentage of the area of ??type II muscle fibers correlation coefficient r = 0.776, P lt; 0.05; intensity exercise group (SHE), apoptotic index and type II muscle fibers The area percentage of the correlation coefficient r 0.737, P lt; 0.05. (3) moderate intensity exercise group cell apoptosis index and MHC Ⅱ a subtype percentage was high positive correlation, the correlation coefficient r up to 0.786, P lt; 0.05; of MHC Ⅱ b subtype percentage was low positive correlation, the correlation coefficient r to 0.254, P lt; 0.05; MHC Ⅱ x subtypes percentage showed a positive correlation, the correlation coefficient r = 0.304, but P gt; 0.05, not statistically significant; MHC Ⅰ subtypes percentage of highly negative correlation, the correlation coefficient r = -0.817, P lt; 0.05 . The results of high-intensity exercise group and the group of moderate-intensity exercise is basically the same. Conclusion: The high percentage of type II muscle fibers of skeletal muscle in the high-intensity treadmill exercise and middle
|