Dissertation > Excellent graduate degree dissertation topics show

Rapid Detection of Down Syndrome by Polymerase Chain Reaction-Simple Sequence Length Polymorphism (PCR-SSLP)

Author: YuDongZuo
Tutor: WangXiuHai;WangPeiLin
School: Qingdao University
Course: Genetics
Keywords: Down syndrome polymerase chain reaction simple sequence length polymorphism detection prenatal diagnosis
CLC: R714.55
Type: Master's thesis
Year: 2002
Downloads: 77
Quote: 0
Read: Download Dissertation

Abstract


Objective To set up a clinical practical method that is highly accurate in rapidly detecting and prenatal diagnosing Down syndrome.Methods The six short tandem repeat (STR) loci onchromosome 21 of 10 families of Down syndrome were detected by polymerase chain reaction-simple sequence length polymorphism (PCR-SSLP). Besides, 4 fetuses were prenatal diagnosed with this method.Results (1) 10 individuals of Down syndrome were diagnosed by PCR-SSLP, the result of which was consistent with that of cytogenetic analysis. (2) The electrophoretic results of 60 STR loci in total of 10 individuals of Down syndrome were analyzed . 30 percent (18 of 60) of them demonstrated a triallelic pattern of three DNA bands, and their ratios of density were 1:1:1; 51.7 percent (31 of 60) displayed a diallelic pattern of two DNA band with ratios of 2:1. (3) The observed heterozygosities of D21S16, D21S11, D21S1432, D21S1437, D21S1270 and D21S1446 were 0.75, 0.70,0.65, 0.75, 0.70 and 0.85 respectively. (4) No mutation was detected in 20 meiosis for the six STR loci. Family analysis of the STR loci confirmed their Mendelian inheritance model. (5) The parental origin of the additional chromosome 21 in 10 individuals with Down syndrome were detected. 9 of them inherited from mother, of which 77.8 percent occurred in meiosis I and 22.2 percent occurred in meiosis II . 1 of them inherited from paternal meiosis I . (6) Two fetuses with Down syndrome were prenatal detected among 4 high-risk fetuses. The results were concordant with karyotyping performed by conventional cytogenetics in all cases. Conclusions The six STR loci specific for chromosome 21 were highly polymorphic, and consistent with Mendelian inheritance model. It is completely accuracy to detect and prenatal detect typical Down syndrome and translocation type of Down syndrome by PCR-SSLP of the six STR loci. Moreover, the operation was simple and rapid. To sum up, the method can be used clinically and it is helpful to etiological research of Down syndrome.

Related Dissertations

  1. Signal Detection Circuit Design for Slow-Light Optic Fiber Gyroscope,V241.5
  2. Research on Automatic Detection Algorithm for Substructure Distress of Highway Pavement Based on SVM,U418.6
  3. Well Detection Pump Steady-Speed Driving System of Brushless DC Motor,TM33
  4. Study on the Echo Processing Method for the Measurement of Wire Length Based on Pulsereflection,TM247
  5. Research on Fault Detection and Network Reconfiguration Algorithms for Distribution Network,TM727
  6. The Matlab Implementation of Joint Source and Channel Decoding of Turbo Code Based on Meanmatch,TN911.22
  7. Research on Autamatic Music Structrue Analysis,TN912.3
  8. Research on 8 Mm-Band Direct Detection Radiometer,TN850.7
  9. Study of Target Simulation and Signal Processing Algorithm for Skywave Over-The-Horizon Radar,TN958.93
  10. Research of Image Mosaic Technology,TP391.41
  11. The Fatigue State Recognition of the Driver Based on Eye Detection,TP391.41
  12. Research on Joint Target Detection for Dual-Sensor Image and System Implementation,TP391.41
  13. Multi-currency Notes Technology Research and Implementation,TP391.41
  14. Camera Calibration and Position and Pose Detecting on Vision Measurement System of PCB,TP391.41
  15. The Research of Moving Object Tracking System Based on Embeded Image Process Unit,TP391.41
  16. Gradual Event Detection in Sensor Networks,TP212.9
  17. Implementation and Research of Illegal Websites Detection System Based on Comparison Methods,TP393.08
  18. Establish a Quantitative Method and Its Kit for Detection of Escherichia Coli and Salmonella. Spp,S154.3
  19. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  20. Research and Improvement of WSNs Routing Algorithm Based on Geographic Location,TN929.5
  21. Research on Detecting Optical Fiber Geometric Parameter Based on Machine Vision,TN253

CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Fetus > Prenatal diagnosis of fetal genetic disorders
© 2012 www.DissertationTopic.Net  Mobile