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The Expression and Significance of Base Excision Repair Gene APE and XRCC1 in Colorectal Carcinoma

Author: ZhaoJingPing
Tutor: LvShen
School: Dalian Medical University
Course: Pathology and Pathophysiology
Keywords: Colorectal cancer Base excision repair APE XRCC1
CLC: R735.34
Type: Master's thesis
Year: 2007
Downloads: 116
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Abstract


Purpose and Background In recent years, with the habits of living of our people, the morbidity and mortality of colorectal cancer showed a clear upward trend. (A) of early colorectal cancer patients after five-year survival rate is almost 100%, with the progression of the five-year survival rate of patients after surgery decreased gradually. Therefore, early treatment is the radical colorectal key. Early treatment is based on early detection and diagnosis. No obvious early symptoms of colorectal cancer is difficult to detect, patients with symptoms such as blood in the stool, the tumor has entered the middle and late, have lost the chance of operation. One important reason for this situation is the pathogenesis of colorectal cancer is unclear, so only the pathogenesis of colorectal depth in order to establish an effective early warning platform, is conducive to the early detection of colorectal cancer. Usually considered DNA molecules inside the cell mutations occur in the physical, chemical and other factors can not be repaired, there is long-term and cumulative tumor. In order to maintain the stability of the genes, biological long-term evolution of the cells to form a variety of DNA damage repair system. Colorectal mucosa cells are genetically unstable cells vulnerable to a variety of toxic substances stimulate DNA damage, if not repaired in a timely manner, the accumulation of mutations prone to malignant transformation. There are five known way to repair DNA damage. Mismatch repair system functions missing part of sporadic colorectal cancer are closely related, then the outside of the mismatch repair repair pathway is associated with colorectal cancer.? Article focuses on the APE gene and XRCC1 genes involved in nucleotide excision repair pathway colorectal cancer. Base excision repair by nucleotide excision repair gene restored normal DNA double helix, thus preventing gene mutation, to maintain genetic stability. APE gene is an important member of the base excision repair, is mainly responsible for the to repair alkylating agents and oxidative damage caused by apurinic / apyrimidinic sites. The XRCC1 gene also involved in base excision repair pathway, the The coded product main role is to repair all damage caused by the single-stranded DNA damage. Currently, less the base excision repair and colorectal cancer reported in the literature. This study was to detect colorectal cancer, adjacent mucosa and normal mucosa APE, XRCC1 protein expression and to explore the mechanism of occurrence of colorectal cancer. Methods colorectal surgical specimens, 185 cases, of which 32 specimens taken adjacent mucosa; normal mucosa of 36 cases. By immunohistochemical methods to detect APE XRCC1 protein expression in cancer, adjacent mucosa and normal mucosa, and analysis of its expression in colorectal cancer patients gender, age, tumor location, differentiation, invasion and lymph node metastasis relationship. All data with SPSS13.0 statistical software for statistical analysis. Results APE protein expression in the nucleus and cytoplasm. Colorectal cancer, of adjacent mucosa APE-positive expression rate was 78.9% (146/185), 81.2% (26/32), both of which are significantly higher than the normal mucosa 61.1% (22/36) (P lt; 0.05), but the former two APE expression difference was not significant (P gt; 0.05) Colorectal APE protein expression and sex, age, tumor location, degree of differentiation, depth of invasion and lymph node metastasis significantly correlated (P gt; 0.05) XRCC1 protein expression in the nucleus. Colorectal cancer, adjacent mucosa XRCC1 positive expression rate was 94.6% (175/185), 87.5% (27/32), both of which are significantly higher than the normal mucosa group 27.7% (10/36) (P lt; 0.05 no significant difference), but the former two XRCC1 expression (P gt; 0.05) Colorectal XRCC1 protein expression and sex, age, tumor location, degree of differentiation, depth of invasion and lymph node metastasis significantly correlated (P gt; 0.05) XRCC1 positive group of colorectal cancer the APE-positive rate was 95.8% (136/142), significantly higher than colorectal the XRCC1 negative group 86% (37/43), XRCC1 and APE both expression showed a positive correlation (r = 0.354 P lt; 0.05). Colorectal cancer, adjacent mucosa, APE and XRCC1 two kinds of protein expression positive rate of 75.8% (140/185), 65.6% (21/32), the two groups were significantly higher than 16.7% of the normal mucosa ( 6/36) (P lt; 0.05), but the difference was not significant (P GT; 0.05) Conclusion 1. Colorectal exist APE XRCC1 expression raised. 2 beside the colorectal mucosa in the presence APE XRCC1 expression increases. 3 colorectal cancer may be associated with DNA replication when the sites of injury and alkylating agents and other toxic substances closely related to injury. Simultaneous detection of colorectal mucosa of different types of DNA repair gene expression contributes to the early diagnosis of colorectal cancer.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms > Colorectal tumors
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