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The high slope pig karyotype with type

Author: LiZhiHui
Tutor: LiuPeiQiong;XuRuHong;LiuRuoYu
School: Guizhou University
Course: Animal Genetic Breeding and Reproduction
Keywords: High slope pigs The sword white miniature pig line Ⅰ Chromosome Karyotype C-band G-banding Ag-NORs
CLC: S828
Type: Master's thesis
Year: 2007
Downloads: 86
Quote: 1
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Abstract


The trial the of Guizhou local varieties of high slope pig for the experimental group , the sword white miniature pig Ⅰ Department for the control group , application of peripheral blood lymphocyte culture technology , and explore the peripheral blood lymphocyte culture its karyotype , C with G banding and Ag -NORs with the best conditions to discuss their similarities and differences , and to provide a basis for the development and utilization of high slope pig and preserving materials , test results are as follows : 1 . White Xiang Pigs Ⅰ Department peripheral blood lymphocytes of high slope pig with sword optimal culture conditions : culture medium pH is 7.0 to 7.2 , to train 68 to 68.5 hours , adding colchicine final concentration of 0.04ug/ml , continue 2 ~ 2.5h, cultured remove to hypotonic treatment for 25 ~~ 30min at the same temperature of the KC1 solution and fixative , air-dried, to obtain good chromosome specimen . 2. Slope pig with sword White Xiang Pigs I diploid chromosome number of other domestic pigs were 2n = 38, karyotype composed : 10sm 4st 12m . 12t . 3. The high slope pig with sword White Xiang Pigs Ⅰ Department of chromosome C Belt sites , consistent with type characteristics , the high slope pig with the sword White Xiang Pigs I C banding frequency showed some differences , high slope pigs 3,5 banding and chromosome 8 higher frequency than the sword white miniature pig line Ⅰ , ⅰ Department show that the high slope swine sword white Xiang Pigs have similarities and differences , can be used as the basis for species identification . 4. G, banding get the high slope pig mid- 54 zone , 212 light and dark bands ; the sword white miniature pig Ⅰ Department 205 bands . G striped number of the high slope pigs sword Department of White Xiang Pigs I are basically the same . 5. The high slope pigs Ag-NORs (2.93 ) below sword of white miniature pig I system ( 3.97 ) , the difference was significant ( P <0.01 ) .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Pig
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