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Cockscomb and Carnation is important ornamental flowers, if we get a new, extraordinary and special new line will undoubtedly have practical value and broad market prospects. The PttKN1 gene as one of KNOX gene family, the ectopic expression of transgenic plants can lead to changes in the leaves, flowers and plant type. Therefore, the use of genetic transformation methods, import PttKN1 gene is a new way to get the new line of flowers. Cockscomb and carnation material by Agrobacterium-mediated genetic transformation method has the PttKN1 gene into these plants, the main results of the phenotypic and molecular levels are as follows: 1. Optimal medium to cockscomb hypocotyl explants regeneration system for the first time through the callus and adventitious buds, adventitious buds: MS additional 6-BA (2.0 mg / l), NAA ( 0.5mg / l) and IAA (0.5mg / l), the differentiation medium adventitious buds frequency up to 33%. 2. Establish a suitable Agrobacterium transformation system, RT-PCR analysis showed: card kanamycin-resistant seedlings have PttKN1 gene expression, and the expression of the gene in the wild-type and tissue culture seedlings, indicating that the gene has been successfully imported The cockscomb middle. 3. , Resulting in transgenic plants leaves phenotypic changes PttKN1 gene overexpression in cockscomb, compared with the wild type, There are three types of change: 1) leaves shrinkage, edge pale bifida; 2) leaves deeply lobed, The volume is reduced, its thickness is twice the original two or three leaves the students; 3) a petiole. 4. Into Carnation, PttKN1 gene kanamycin resistant seedlings appeared in a variety of phenotypes, there are significant differences in the phenotype of the wild-type plants, its main types: 1) the phyllotaxis change: wild-type plants usually was for health phyllotaxy; transgenic plants three whorled phyllotaxis four whorled phyllotaxis and more whorled phyllotaxis phyllotaxy irregular change between the Health and alternate phyllotaxis performance the leaves fascicled; 2) stem shape change: wild-type stems nearly circular, and transgenic plants appear thicker flat stem; 3) plant morphology change: dwarf tufted plants. These results suggest that, PttKN1 gene into cockscomb and ectopic expression of carnation caused many changes in leaf and plant morphology of transgenic plants, ornamental plant lines improved to provide extremely valuable genetic resources, and also that PttKN1 gene as a transcription factor may regulate the expression of other genes, plays a very important role in plant morphogenesis and development.
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