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Objective: To observe the topoisomerase I inhibitor ( topotecan ) the destruction strains of L1210/ADM cells and induction of apoptosis . Methods: MTT assay , Annexin V staining , DNA ladder electrophoresis cell morphology observation method of analysis of topotecan the killer role of Kang L1210/ADM cells , apoptosis , caspase-8 inhibitor IETD - FMK , DEVD - CHO topotecan the Kang -mediated target cell killing, apoptosis and caspase . Results : TPT the L1210/ADM cell lines with a strong killing effect , but also has significant time-dependent , the same time, the performance of the target cells phosphatidylserine externalization, apoptotic bodies ladder electrophoresis . Add a specific inhibitor of caspase enzyme group at each time point strains of L1210/ADM cells survival rates were higher than with topology irinotecan group ( P lt ; 0.05 ) , related apoptosis was not obvious . Conclusion: Topotecan the L1210/ADM cell lines with strong killing and induction of apoptosis , the mechanism may be related to caspase - 8 , the order of activation .
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