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[Objective] To seek a source of extensive , inexpensive method of establishing stable and viable hepatitis B virus associated glomerulonephritis mouse model . 10 【 Methods】 model and control groups of BALB / c mice each individual mouse urine protein indicator is normal , model group mice by intraperitoneal injection of clinically diagnosed patients with hepatitis B positive serum observed after one week , two weekly , continuously injected around, and after a week since the start of injection twice to test strip method to measure urinary protein in mice in the control group to the same amount of 0.9 % saline instead of HBeAg serum . Dealing with the model group . The fifth week of the control group and the model group mice kill all whichever kidney tissue do light microscopy, immunofluorescence and transmission electron microscope . 【Results】 fifth weekend all model mice urine protein positive light microscope lesions , most of mesangial proliferative glomerulonephritis, the severity of mild to moderate , some severe lesions in mice . And visible deposition of immune complexes . Deposition sites of glomerular mesangial area , subendothelial . Immunofluorescence showed that all model mice HBsAg is positive, and the strength of - . Electron microscope model mice visible immune complex deposition , deposition in the mesangium and subendothelial , and the results of light microscopy . Control mice not found the lesions . [Conclusion] The experimental model mice urine protein positive light microscope mesangial light , moderate and severe proliferation, immunofluorescence HBsAg strength - . Electron microscope showed that the immune complex deposition in the mesangium and subendothelial has been basically in line with the current hepatitis B diagnosis of kidney conditions , the model is successful .
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