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Study on Association of Calpain 10 Polymorphism with Type 2 Diabetes Mellitus in Uigur Population in Xinjiang

Author: ZuoZhiHua
Tutor: XieJianXin
School: Shihezi University
Course: Biochemistry and Molecular Biology
Keywords: Type 2 diabetes mellitus TNFα gene Calpain10 gene Single nucleotide polymorphisms Xinjiang Uygur
CLC: R587.1
Type: Master's thesis
Year: 2009
Downloads: 15
Quote: 0
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Abstract


Objective: This study mainly by detecting TNFα 308 G → A mutation and Calpain10 a gene (SNP43G / A, SNP63C / T) locus polymorphism in to investigate the TNFα 308 G → A mutation and Calpain10 gene polymorphism the relationship of the Xinjiang Uygur type 2 diabetes genetic susceptibility. : Uighur type 2 diabetes patients and normal volunteers were digest Endocrinology, collected 120 patients with type 2 diabetes and 120 cases of hospital patients and normal controls in a 1:1 ratio, from the People's Hospital of Xinjiang Uygur Autonomous Region outpatient blood samples of healthy persons, serum TNFα levels using enzyme-linked immunosorbent assay (ELISA), molecular biology detected by PCR-RFLP TNFα 308 G → A the variability and Calpain10 gene locus (SNP43, SNP63) polymorphism The distribution of genotypes and gene polymorphism analysis. SPSS13.0 statistical software, relative risk, odds ratio (Odd ratios, OR) and its 95% confidence interval (Confidence interval, CI) and compare with type 2 diabetes patients and controls TNFα 308 points and Calpain10 of polymorphism of the gene (SNP43, SNP63) distribution of the statistical treatment of the data using χ2 test, and test whether the genotype distribution to conform the Hardy-Weinber balance's law. Results: (1) Case serum TNFα levels average (28.66 ± 15.28) pg / mL; TNFα levels of the control group average (22.73 ± 14.24) pg / mL, the patient group was significantly higher serum levels of TNFα difference statistically significant (P lt; 0.05). (2) of TNFα three genotypes of 308 points of distribution in case / control group: the the GG type 89/104 (74% / 87%); GA AA-type 31/16 (26% / 13% of); distribution of the A allele in both groups: the G allele 209/224 (87% / 93%); A allele 31/16 (13% / 6%). TNFα-308 polymorphism genotype and allele frequency differences in the distribution of the two groups, the difference was statistically significant (P lt; 0.05). (3) TNFα different genotypes between serum levels of TNFα were: the GG genotype (22.11 ± 2.23) pg / mL GA AA type (29.78 ± 3.26) pg / mL GA AA-type level higher than the GG genotype difference statistically significant (P lt; 0.05). (4) SNP43 polymorphism loci three genotypes distribution in case / control group: the GG type 101/86 (84% / 72%); GA 16/26 (16% / 22%), AA type 0/8 (0% / 6%); the G alleles A distribution of two groups: the G allele 221/198 (92% / 83%); A allele 19/42 ( 8% / 18%). The SNP43 polymorphism locus genotype and allele frequency differences in the distribution of the two groups, the difference was statistically significant (P lt; 0.05). (5) SNP63 polymorphism loci three genotype distribution in case / control group: CC 61/62 (51% / 52%); CT 42/33 (35% / 28%), TT 17/25 (14% / 21%); C and T allele distribution in both groups: the C alleles 164/157 (68% / 65% of); T allele 76/88 (32% / 37%). The the SNP63 polymorphism genotype distribution and allele frequencies in the two groups no difference was not statistically significant (p gt; 0.05). Conclusion: (1) serum TNFα levels in Xinjiang Uygur population with type 2 diabetes were positively correlated; (2) TNFα section. 308 G → A variation with the Xinjiang Uighur population in type 2 diabetes; (3) Calpain 10 gene SNP43 polymorphism with the Uygur population with type 2 diabetes have a significant correlation; (4) Calpain 10 gene SNP63 polymorphism loci the Uygur population with type 2 diabetes was no significant correlation.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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