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Objective: To study bone morphogenetic protein-7 (bone morphogenetic protein 7, BMP7) on the in vitro culture of high phosphorus-induced rat vascular smooth muscle cells (VSMC) calcification. Methods: Application rat aortic smooth muscle cells in vitro experiment, rats were divided into 3 groups: the normal phosphorus concentrations (Pi1.4mmol / L) group, high phosphorus the concentration (Pi2.4mmol / L) group and high phosphorus BMP7 group, respectively, cultured for 3, 6 and 9 days. The medium was changed the day D0 days. Application BCA assay intracellular calcium content of different times, radioimmunoassay (RIA) method was used to detect the culture osteocalcin content by ELISA the different time nuclear binding factor α1 (Core Binding Factorα1, Cbfα1) DNA binding activity, Immunohistochemical The chemical used to detect the expression of alpha smooth muscle actin (α-smooth muscle actin, α-SMA). Results: the normal phosphorus concentrations VSMCs calcium content in 9 days no significant change, high phosphorus concentrations VSMCs calcium content in D3, D6, D9 D0 significantly increased (P lt; 0.05), high phosphorus BMP7 (200ng/ml) group D6 and D9 cell calcium content compared with the same time high phosphorus concentrations were significantly lower (P lt; 0.05). After 72 hours of incubation, compared with normal phosphorus concentrations supernatant osteocalcin level, high phosphorus concentrations of osteocalcin levels increased significantly (14.62 ± 2.93pg/μg protein vs of 2.83 ± 0.64pg/μg protein, P <0.01 ). BMP7 group supernatant osteocalcin high phosphorus content of 9.36 ± 1.373 pg / μg protein, the difference was significant (P lt; 0.05), compared with the high phosphorus concentrations. High phosphorus concentrations in D3, D6, D9α-SMA percentage of positive cells compared to normal phosphorus concentration was significantly reduced (P lt; 0.05), and percentage of positive cells gradually decreased with time; BMP7 group of high phosphorus in D6 d9α-SMA-positive cell percentage compared with the same time high phosphorus concentrations increased significantly (P lt; 0.05). Cbfa1 activity of normal phosphorus concentration of cells in 9 days no significant change. Compared with D0, high phosphorus concentrations in the D6 and D9 days of cell Cbfa1 activity increased significantly (P lt; 0.05). BMP7 group of high phosphorus D6 and D9 cells Cbfa1 activity and at the same time high phosphorus concentrations are significantly lower (P lt; 0.05) Conclusion: High phosphorus directly stimulate the rat VSMC calcium deposition and osteocalcin induces the production increase, and its role may be associated with high phosphorus increase VSMC Cbfa1 expression; BMP7 inhibited rat VSMC calcium deposition and osteocalcin generated, its role may be related to the decrease VSMC Cbfa1 expression.
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