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Expression and Significance of Heat Shock Protein 70, 90 in Human Normal Endometrium, Endometrial Hyperplasia and Carcinoma

Author: BaiXiaoXia
Tutor: ChenYaQiong；XinXiaoYan
School: Fourth Military Medical University
Course: Obstetrics and Gynaecology
Keywords: Endometrial Heat shock protein Immunohistochemistry Cell culture Image Analysis
CLC: R737.33
Type: Master's thesis
Year: 2003
Downloads: 87
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Abstract


Objective: HSP70, 90 is a chaperone of the steroid hormone receptor (SR), its expression and changes affecting the structure and function of the SR, and the neointimal growth lesions closely related. In this study, the determination of endometrial tissue in HSP70, 90 expression and ER expression to observe the impact of estrogen on the expression of normal endometrial epithelial cells in vitro culture of Hsp70, 90, research in the womb Expression and significance of membrane normal, hyperplasia and cancer tissue, to provide experimental evidence for the diagnosis and treatment of endometrial lesions and new ideas. Methods: (1) the use of immunohistochemistry and image analysis to detect the expression of HSP70, 90 normal human endometrial hyperplasia and cancer tissues. (2) enzyme digestion Isolation, culture and identification of normal endometrial epithelial cells. (3) using the above method to detect and analyze the estrogen from the body endometrial epithelial cells expression of HSP70, 90. Results: (1) human endometrial tissue the Hsps positive signal for epithelial and stromal cells cytoplasm and / or nucleus brown yellow granules. Hsp70 in normal endometrial hyperplasia cytoplasm mainly endometrial cancer in the cytoplasm and nucleus expression HSP90 in the cytoplasm of all organizations are strong nucleus expression of ER-positive signal epithelial and / or stromal cell nuclei appear brown particles, focal or diffuse distribution of positive cells. (1) normal endometrium, secretory endometrium, endometrial proliferative phase of HSP90, ER expression increased (P skill 5), the expression of HSP70 the weakened (skill). m endometrial hyperplasia, HSP70, 90, the ER three proteins between simple and complex group no significant difference compared with normal proliferative and secretory endometrium, HSP90, ER expression increased (P < knife 5), HSP70 expression did not change significantly (P> 0.05). O) HSP70 expression in endometrial cancer stronger than the proliferative phase, secretory phase and hyperplasia endometrium (P <0.05 \\ Hsp90, ER expression below the proliferation of endometrial hyperplasia and endometrial (<0 knife 5X ( ) endometrial cancer HSP70 expression enhanced with pathological grade (GI, GZ, G3) increased (P <0 * 5), HSP90 expression increased with the pathological grade and weakened (P <0 * 5X and non-endometrioid gland cancer (adenosquamous carcinoma the translucent cell carcinoma and papillary serous adenocarcinoma), endometrial adenocarcinoma HSP70 expression diminished (P <0 * 5), HSP90 expression increased (P <0.05); endometrial cancer, the expression of HSP70, 90 and clinical stage, lymph node metastasis and depth of myometrial invasion was no significant correlation (P> O knife 5). clever) HSP90 expression in endometrial ER expression was positively correlated HSP70 expression of ER expressed as a negative correlation. door) estrogen stimulation of HSP90 expression in endometrial epithelial cells, inhibition of Hsp70 expression, estrogen receptor inhibitor (IC 82780) on HSP90 inhibition of estrogen stimulation, but does not affect the effect of estrogen on inhibition of HSP70 Conclusion: door) Hsp90 and ER expression in different endometrial consistency prompted H there is a dependency on the function and organization of HSP90 inhibition or antagonist and / or expression of ER can inhibit the effect of estrogen on stimulation of the growth of endometrial cells to provide new ideas for the treatment of endometrial hyperplasia and endometrial cancer. p) endometrial tissue expression of Hsp70 expression of ER in the opposite trend, the impact of estrogen on the expression of Hsp70 non-ER-dependent, suggesting that endometrial expression of HSP70 is not entirely under ER regulation. O) HSP90 expression in endometrial carcinoma is lower the higher the expression of HSP70 worse, cell differentiation, the higher the degree of malignancy, therefore HSP70, 90 expression can be used as biological indicators to predict the prognosis of patients; Hsp90 expression in hormone on cell proliferation stimulation, the expression of HSP70 has a protective effect on the cell damage, inhibition of HSP70 in 90 expression could inhibit cell proliferation and induce apoptosis, in combination with the chemotherapy drugs may improve the outcome.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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