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Objective: To establish the mouse hyperuricemia acute, chronic model to explore Qinpi total coumarin (TCCF) impact of hyperuricemia serum uric acid levels in mice, and to explore its mechanism of action. Method: 1. Mice by intraperitoneal injection of different doses of potassium oxonate, explore ways to establish the optimal dose mice acute hyperuricemia model; using xanthine potassium oxonate, adenine, potassium oxonate, adenine ethylamine butanol, xanthine ethambutol four methods of modeling, to explore the establishment of the mouse chronic hyperuricemia model. Given different doses in mice TCCF, to explore TCCF of mice acute hyperuricemia BUA, XOD, ADH, GDH. 3. Give mice with different doses of TCCF, of explore TCCF the mouse chronic hyperuricemia BUA, XOD, UUA, CR, BUN. Results: 1. Potassium oxonate significantly elevated in mice BUA levels, but a short time greatly increased BUA level of mouse liver, renal injury, so use potassium oxonate in doses of 250 mg / kg intraperitoneal injection of mice with acute hyperuricemia model. Xanthine of 600mg/kg (ig.) oxonic potassium 1OOmg/kg (sc.) the BUA mice, CR and BUN values ??significantly higher than the control group, so this method to create mice with chronic hyperuricemia model . 2. TCCF significantly reduced in mice with acute hyperuricemia BUA values, the middle dose group best the; TCCF of XOD acute hyperuricemia mice was significantly inhibited, the highest dose inhibition rate; TCCF on mice acute hyperuricemia ADH, GDH activity had no significant effect. 3. TCCF significantly reduced in mice with chronic hyperuricemia BUA value, the effect of high-dose group; the UUA of TCCF mice was significantly lower than the model group UUA values ??of the middle dose group with the control group showed no statistical the significance; TCCF effective inhibition of XOD chronic hyperuricemia mice, high-dose inhibition rate; TCCF, middle dose group CR, BUN value was slightly lower than the model group, but not statistically significant. Conclusion: 1. Intraperitoneal injection of potassium oxonate 250 mg / kg can establish an effective, safe mouse acute hyperuricemia model; the the xanthine 600mg/kg (ig.) the potassium oxonate lOOmg / kg (sc. ) to establish the most stable, continuing mice with chronic hyperuricemia model. 2. TCCF significantly reduce acute and chronic hyperuricemia mice BUA level, the mechanism of action may be related to the inhibition of hepatic XOD activity. 3. TCCF does not aggravate renal damage, preventive and protective role of uric acid nephropathy.
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