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Senile dementia,that is Alzheimer’s disease (AD),is a chronic, neurodegenerative disease, which is characterized by the impairment of memory and cognition as the main clinical features. So far there is no completely effective method to treat AD, especially the advanced AD, in that at this time the brain damage has entered an irreversible phase and led to poor treatment. Mild cognitive impairment (MCI) is widely recognized as the pre-dementia state, also the former state of the Alzheimer’s disease, thus in-depth study to MCI is expected to provide the best treatment time to prevent and delay AD.As been widely accepted,β-amyloid protein (Aβ) plays a central role in AD pathogenesis. The abnormal release and deposition of Aβis the initiating factor to form senile plaque and the important components of its core. In the injury cascade reaction of AD’s nerve cell, typical changes of the pathophysiology, such as amyloid precipitation,have occurred before the abnormal phosphorylation of tau protein. Be-sides,abnormal metabolism of Aβis one of the reliable biological indicators to show the conversion of MCI to AD.Lipid metabolism may lead to cerebral arteriosclerosis,which will be able to change the brain microenvironment, reduce cerebral blood flow and damage to brain metabolism, resulting in the cognition of the aged declines. So the increase of serum cholesterol is an important and harzardous factor for MCI, and the evidence that high blood cholesterol disease could cause Aβdepositing had been found. Aβaggregating can generate the inflammatory reaction by the pathway of complement, and there have been researches to suggest that central inflammation is the early feature of AD’s pathological changes. Moreover, rigorous regression analy-sis showed that the level of the interleukin-6 (IL-6) in the plasma increasing had related to the damage of the cognitive function closely, thus it is a risk factor leading to cognitive impairment.Therefore, the therapeutic measures, decreasing the generation and aggregation of cerebral Aβ, lowering the level of blood fat to improve the brain microenvironment, increasing cerebral blood flow to maintaine normal brain metabolism and lowering the level of IL-6 in plasma to block the inflammation cascade effects of Aβ, have particular significance in the early treatment to MCI.Tutor Professor Yuan Depei, according to his clinical experience in many years, has proposed a method that strengthening our foundation and brain to treat the MCI, and created this physic liquor and the corresponding plus and minus prescription, whose clinical application has been proven quite effective to improve learning and memory ability significantly and raise the aging integral value of the TCM testimony, moreover, without any side effects. And preliminary experimental study had also confirmed that the physic liquid can be reduce blood viscosity in aged rats and increase the activity of their erythrocyte SOD with a placid way to improve the cellular immune function and against the atrophy of spleen and thymus. Furthermore, this physic liquor could obviously increase the content of noradrenaline (NA) and dopamine (DA) in the brains of experimental animals, and significantly inhibit monoamine oxidase (MAO) and the vitality of acetylcholinesterase (AchE) to pro-mote the learning and memonic ability of laboratory animals.Objective: This topic was based on the early research of the consolidating foundation and strengthening brain method. To establish experimental animal model in accord with the praxiological and pathological characteristics of MCI;thereby, the mechanism of the consolidating foundation and strengthening brain method to enhancing the learning and memonic ability had been more in-depth revealed, which provided a more extensive basis to the clinical application of this method. Methods:①With systematic review of the literature research methods, analyze the theoretical basis of consolidating foundation and strengthening brain method in treating MCI.②To built MCI model of rats by the way of intra-peritoneal injection of D-galactose and NaNO2.Then,divided into high, medium and low dose, injected into the model animals, and set up the normal control group, and piracetam control group to observe the impact on the Rat Behavior by water maze test, Aβexpression of hippocampus with immunohistochemical staining-method,the level of the serum cholesterol, using routine methods, etc. and the content of serum IL-6 with ELISA.Results:①The subside of learning and memonic ability, related to five internal organs, six hollow organs, house of Qi Heng, Qi, blood, body fluid, Jing, meridian, and so on. But the most important is the deficiency of spleen and kidney.②Behavior change: the model rats,whose escape latency in the water maze and the time of searching path length and crossing the platform with the same number, was significantly longer than the normal control group (P<0.05). But the treatment of both the consolidating foundation and strengthening brain liquor and piracetam could make the index above improved significantly (P<0.05), even the high and medium dose group of our liquor appeared better treatment (P <0.05).③Aβimmunohistochemistry results:The expression of model group in hippocampus of Aβincreased significantly compared with the normal group, and all the treatment group could reduce the expression of Aβin the hippocampus, but the effect of both the low dose group of our liquor and Piracetam group is not obvious.④The level of serum cholesterol and the content of serum IL-6 changes: The level of serum cholesterol and the content of serum IL-6 of model group increased, significantly higher than the normal group (P<0.05).And our liquor, in spite of any dose, could reduce the level of blood fat and serum IL-6, besides, the effect of high and medium dose group was better. Piracetam group could reduce the level of serum cholesterol effectively (P<0.05), but with a poor effect to lower the level of serum IL-6.Conclusion: These results suggest that:According to the method of our consolidating foun-dation and strengthening brain to prescribe,can not only promote the learning and memonic ability of the rats with MIC significantly, but also reduce the generation and accumulation of Aβin brain of experimental animals and decrease the levels both the blood fat and Serum IL-6 to improve the brain microenvironment, which can prevent the conversion of MCI to AD effectively. From tests above, a conclusion can be drawn that tutor Professor Yuan Depei’s realization that the spleen and kidney deficiency, brain dystrophy, and stagnant phlegm led to cognitive impairment is reasonable, and the consolidating foundation and strengthening brain method is an effective treatment to MCI.
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