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Objective: To observe experimental arthritis in rat plasma tumor necrosis factor (TNF-α), interleukin-1 (IL-1), prostaglandin 2 (PGE2) content, explore the TNF-α, IL-1 and PGE2 mechanism in RA; observed the Three Tigers pill on experimental arthritis in rat plasma TNF-α, IL-1, PGE2, the impact of explore the Three Tigers pill treatment of RA mechanism of action. Methods: 32 SD rats were randomly divided into four groups: ① normal control group (normal group, n = 8); (2) the model control group (model group, n = 8); In treatment group ③ of kidney Juanbi Pill (YSJBW group, n = 8); ④ three tiger pill treatment group (SHW, n = 8); model group, Yi Shen Juan Bi pill group, three tiger pill group left rear foot pad subcutaneous injection type Ⅱ collagen (collagen Ⅱ, C Ⅱ) plus fully blessing 's adjuvant-induced experimental rheumatoid arthritis model, normal group were not the injections. Pills group and the kidney Juanbi pill group Three Tigers after the initial immunization 7d drugged the three tiger pill and the kidney Juanbi pill, twice a day, the model group orally daily 2ML distilled water; the normal group allowed free access to water. Medication for 14 days, the normal group, model group, the three tiger pill group of kidney Juanbi Pill rats in modeling 2l days decapitated after blood was detected by radioimmunoassay, plasma TNF-α, IL-1, PGE2, content . Arthritis exponential integral method to evaluate the symptoms of arthritis. Results: (1) general observation: the course of the experiment, the normal control group rats coat is white and shiny, lively, diet, stool normal. Rats modeling performance body hair less shiny, mental, and eat less, which is the most obvious model group. (2) arthritis symptoms observed: 24 rats have showed arthritis symptoms, after modeling 2d visible the primary swelling the rat left proinflammatory foot reached the peak at the right of the part of the rat model about 9 days non-induced inflammation foot secondary arthritis symptoms, secondary swelling stabilized around 14 days. (3) Arthritis Index Points Rating: model group, the three tiger pill group and kidney Juanbi pill rats with the immune prolonged, arthritis index points increase in the 14d was no significant difference between the three groups, but after 17d model group than that the three tiger pill group and kidney Juanbi of pill group (p lt; 0.01), the model groups arthritis exponential integral slow downward trend. (4) RIA test results: after modeling 21d, the model group three tiger pill group, and the the the kidney Juanbi pill group TNF-α, EL-1, of PGE2 content was significantly higher than the normal group (P lt; 0.01), three Tiger Pills group, the the kidney Juanbi pill group TNF-, IL-1, PGE2, content was significantly lower than the model group (P lt; 0.01 or p lt; 0.05) the three tiger pill group the kidney Juanbi pill group TNF-, IL- of PGE2 content difference was not statistically significant. (P gt; 0.05). Conclusions: (1) the left foot pad subcutaneous injection of bovine C II arthritis symptoms in four groups of rats, animal models of RA successfully copied. (2) in RA of TNF-, IL-1, PGE2, promote the formation and development of synovitis, and may affect each other, mutual promotion and coordinating role, one of the important mechanisms for the pathogenesis of RA. (3) Three Tigers pills lower plasma TNF-, IL-1, PGE2, content, Three Tigers pill can prevent synovitis Inhibitory synovial pannus formation may be the treatment of RA, one of reflects the efficacy of the mechanism; Toramaru blocking of TNF-, IL-1, the PGE2 effect kidney Juanbi pill group showed no significant difference.
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