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Construct Tissue-engineered Bone by Co-seeding Marrow Stromal Cells and Endothelial Cells

Author: ZhouJiang
Tutor: ChenHuaiQing
School: Sichuan University
Course: Biomedical Engineering
Keywords: Rat bone marrow stromal cells Human umbilical vein endothelial cells Bone Tissue Engineering Biodegradable materials
CLC: R318.1
Type: Master's thesis
Year: 2003
Downloads: 70
Quote: 0
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Abstract


Bone tissue engineering is the use of tissue engineering principles for bone tissue defect repair or rebuild. Since the use of bone tissue engineering bone graft substitutes has been constructed, the researchers found that the bone graft into the role of slow, can not match the scaffold degradation, mainly for two reasons: (1) the role of seed cells into bone slow; ( 2) nutrient vessels grow slowly, due to the capillary network within the material grows very slowly, because of lack of nutrient supply of seed cells can not crawl deep into the material, making the role of an effective substitute for bone deposition degradable biomaterials. In recent years, researchers found that endothelial cells can secrete bone morphogenetic protein (BMP), to promote osteogenic differentiation and stimulate osteoblast precursor cells and the secretion of vascular endothelial growth factor (VEGF), and VEGF can promote endothelial cell proliferation and angiogenesis, also has a significant role in osteogenesis. Thus, we can assume that if the bone marrow stromal cells and endothelial cells grown in bio-composite materials combined on bone tissue engineering, it may promote osteogenesis at the same time accelerate local angiogenesis and osteogenesis process to meet the nutritional needs. In in vitro experiments, we found that endothelial cells under the action of bone marrow stromal cells (experimental group) to form calcified nodules was significantly more than the untreated bone marrow stromal cells (control group), and in the culture section 14d and 21d, the experimental group of alkali phosphatase (ALP) activity and osteocalcin (OCN) secretion in approximately 2-3 times higher than that of endothelial cells to bone marrow stromal cells have contributed to the role. In vivo, we'll rat bone marrow stromal cells and human umbilical vein endothelial cells grown in L-polylactic acid / β-tricalcium phosphate (PLLA / β-TCP) porous scaffold material as the experimental group, bone marrow stromal cells grown in The material used as the control group, the experimental group capillary network formation within the material to be significantly better than the control group, but significantly increased bone osteogenesis version stromal cells and endothelial cells combined with planting build Gimli denier woven bone mouth two-way cloth open study a life positions digest faster, suggesting that bone marrow stromal cells and endothelial cells combined grown in scaffolds tissue engineered bone conducive material internal vascular network formation, it is possible to solve the seed cells due to lack of nutrient supply and can not climb into the material deep into bone problems.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Artificial organs and organ
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