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Serum anti-neutrophil cytoplasmic antibody (Anti-neutrophil Cytoplasmic Antibodies, ANCA) are a class of particles for the main components of neutrophils and monocytes lysosomes autoantibodies. Based on indirect immunofluorescence staining is divided into different parts of neutrophil cytoplasmic (C-ANCA) and perinuclear (P-ANCA) two kinds. The main target cytoplasmic antigens protease (Proteinase, PR-3); perinuclear major target antigen is myeloperoxidase (Myeloperoxidase, MPO). In the past that ANCA is Wegener's granulomatosis (Wegener's Granulomatosis, WG) as the representative of systemic necrotizing vasculitis serum marker antibodies, as indicators of the diagnosis and assessment of disease activity indicators, remission, relapse has certain predictive value. As in many other diseases are detected ANCA, autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, scleroderma, dermatomyositis, and thus are not considered serum ANCA are associated with systemic vasculitis obvious correlation, but also with autoimmune diseases also have some relevance. PATIENTS AND METHODS 122 cases were hospital clinic or ward (2003.1 ~ 2004.1) of patients. Divided into two groups, connective tissue disease group of 84 cases, including 36 cases of systemic lupus erythematosus (including 19 cases of lupus nephritis), rheumatoid arthritis, 16 cases, 12 cases of dermatomyositis, scleroderma eight cases, dry integrated sign eight cases, three cases of overlap syndrome, mixed Zhejiang University master's degree thesis Session 2 side lining connective tissue disease in 1 case. Cutaneous vasculitis group of 38 cases, including 14 cases of allergic purpura, erythema nodosum 12 cases, seven cases of allergic cutaneous vasculitis, Behect disease in 5 cases. All patients met the \30 patients in our hospital health volunteer blood donors. By indirect immunofluorescence (IIF) assay ANCA. Kit was purchased from Germany, Europe and Mongolia Company. The patient sera, normal sera o.olmof several PH7.4 phosphate buffer (P BS.Tween) l: add diluted dropwise 25ul absorb the bio sheet at room temperature for 30 and n, let PBS.Tween water washing buffer to remove unbound sample, and then immersed in PBS. claw pillow and n en solution for 5 or more. Add 20ul fluorescent labeled anti-human Ig. Incubated for 30min, dark reactions. Washed with PBS.Tween unbound antibody. Then immersed in the solution for 5 PBS.Tween than n. After washing, glycerin mounted. Fluorescence microscope to neutrophils is specific positive green fluorescence is determined, divided by the fluorescence intensity of staining ~. Fluorescent form in two ways: like cytoplasmic granules along uneven coloring and leaf between heavy nuclei stained nucleus were called cytosolic (C.ANCA), around the nucleus is called perinuclear coloring Iraq an ANCA). Results and discussion paper ANCA positive staining were perinuclear ANCA (P.ANCA), connective tissue disease group, the positive rate was 46.4% in the group of cutaneous vasculitis positive rate was 13.2%, the normal control group were negative, disease group and normal control group there were significant differences between the comparison (P lt; 0.001 and P lt; 0.05). 84 cases of connective tissue disease, systemic lupus erythematosus (Syste while cL that usE mouth th ennatosus, sLE) 36 cases were positive for 20 cases (positive rate of 55.6%); rheumatoid arthritis twisted address diffuse hundred city. idAd'tis, RA) 16 cases were positive in 8 cases (positive rate was 50%), which was significantly longer than patients with negative history, severe illness, and many are active disease; dermatomyositis mouth ~ atomyosids, DM) 12 例The positive rate of 16.7%; systemic scleroderma mouth while shooting g limit ssive Syste. Scleroderma, PSs) 8 cases, the positive rate of 25%; Dry parade syndrome (Sjog limit ns \cases, the positive rate \yl. wonderful time oidh knock gas tanks) were 14 cases, Zhejiang University, two master's degree thesis on a monthly session of serum ANCA positive in 2 cases, the positive rate was 143%; erythema nodosum Tam Doo also ema Nodo ~, EN) 12 cases, positive in 2 cases, the positive rate of 16.7%; allergic cutaneous vasculitis (Alle worthy ic CutsLncous vasculitis, Aev) 7 cases, positive l cases, the positive rate of a 4.3 percent; followed by Behcet's disease (Behect'5 Disease, BD ), 5 cases were negative 36 SLE patients, ANCA-positive, 20 cases are in active disease, including 15 cases of ANA seven 1:80 for high-titer, 17 cases of anti-ds.DNA antibody (), 9 cases of anti-Sm antibodies (), 20 patients with elevated ESR all, 17 cases decreased C3, C4 dropped 14 cases, while 16 patients with ANCA-negative, only six cases of AN Gun 1:80; 8 cases of anti-ds DNA antibodies a (), 8 cases of anti-Sm antibodies (), 12 cases of elevated ESR, 6 cases decreased C3, C4 five cases dropped. statistical processing and display, ANCA-positive and negative groups are compared, for tips SLE disease activity indicators such as ANA Diao: 80, anti-ds.DNA antibodies, erythrocyte sedimentation rate (ESR) to accelerate and complement C3, C4 and decreased between the two groups, there were significant differences, suggesting that ANCA detection of SLE disease activity state has a certain significance. 36 cases of SLE Unconsolidated lupus kidney patients 17 cases, including 3 cases of ANCA-positive, weakly positive in 3 cases positive rate of 35.3%; 19 cases of lupus kidney patients, ANCA strong positive n cases, positive in 3 cases, the positive rate 73.7%. been xZ test, P lt; 0.05, there are significant differences. Conclusions 1. ANCA in connective tissue disease and cutaneous vasculitis positive rates were 46.4% and 15.8%, suggesting that these two sets of ANCA in the pathogenesis of the disease have a role. 2.ANCA detection of SLE disease activity monitoring and determine whether the combined kidney damage has a certain significance. 3.ANCA evaluate RA disease duration as its severity as an indicator.
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