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Diabetes and its complications serious threat to human health, a global public issues. The cardiovascular system diseases diabetes mellitus serious threat to the health of patients with diabetes. Through its concurrent diabetes macrovascular disease, microvascular disease and autonomic neuropathy which affects the cardiovascular system. Objective: Diabetic cardiomyopathy is currently considered an independent heart disease. Diabetes is the common cause of cardiac dysfunction, then the occurrence of diabetes in the end how the myocardial lesions? Here in the electron microscope is how to behave? And these lesions can use drugs to intervene. PPARgamma agonists - also known as Avandia rosiglitazone (thiazolidinediones, TZDs) is an insulin sensitizer, it is currently a lot of research and found that in addition to its hypoglycemic protect the cardiovascular system than there are role. And this kind of rosiglitazone myocardial protection mechanisms are not very clear, after treatment on cardiac structure and how to change at home and abroad has been reported. This study hopes to establish diabetic cardiomyopathy model after treatment with rosiglitazone compared with the control group, some of the results obtained. For the clinical treatment of diabetes and diabetic cardiomyopathy provide experimental basis. MATERIALS AND METHODS: The model of diabetic cardiomyopathy: a. Purchase of 35 10-week-old Sprague-Dawley (sD) rats (by the Experimental Animal Center of Zhejiang University) weighing 313 ± 46 g, 2. Streptavidin STZ (Streptozotocin, STZ); protamine zinc insulin injection; ketamine hydrochloride Zhejiang University master's degree thesis needle; phenobarbital needle; 3.SD rat grouping processing: the experimental group (male, 10 , female, clever only), 60mg/kg intraperitoneal injection of STZ 55 a rafter structure of 0.IM pH4.5 sodium solution 3ml (diabetic group). The diabetic model, in which the male and female of the five. On the third day since rosiglitazone with lmg / kg / d orally (rosiglitazone). The control group (male, 5, female, 5), intraperitoneal injection of sodium liquid 0.IM pH4.5 constitutive rafters 3ml; 4. Animal Feeding: All SD rats in terms of cleanliness (two) reared under conditions of food and water can freely get; model after two days, the daily urine test. If the urine one, not treated, urine, ten ten ten, then subcutaneous protamine zinc insulin a fly units / day; 5 rats treated: model 10 weeks after intraperitoneal injection of anesthesia blood extraction chamber, a blood glucose meter for fast method (U.S. Roche Adantage) blood glucose. Meanwhile killed after myocardial specimens fixed with 2.5% glutaraldehyde; 6. Statistical analysis: Data are expressed as mean ± standard deviation S x said, using two sample t test. If P lt; 0.01, the difference is highly significant, as P lt; 0.05, the difference is significant. TEM Sample Preparation: 1. Tissue fixation, washing, dyeing, dehydration. 2 embedded. 3 slices (semi-thin sections and repair blocks and thin sections). 4 fishing tablets: with 200 mesh copper (copper mesh by ultrasonic cleaning with acetone) fishing film. 5 ultrathin sections stained. 6 Netherlands, Philips TECNA10 TEM. Zhejiang University master's degree thesis results: starting from the day after the injection sTz experimental rats were appeared polydipsia, polyuria, polyphagia, increased urine is greater than. However, there are five diabetic female rats, as early as three weeks urine, ten, seven weeks after a urine, soil or, saying it five female rats diabetic subgroup. A body weight of rats: the 10 weeks ended, diabetic group (female, male) and the control group, body weight decreased significantly. Rosiglitazone group (female, male) and the control group, weight decreased. Diabetic sub-group and the control group, body weight declined slightly. Two rat glucose: the 10 weeks ended, diabetic group (female, male) and the control group, blood glucose was statistically significant. Rosiglitazone group (female, male), diabetes subgroup and the control group, no significant blood sugar. Three. Myocardial ultrastructure: (l) normal rat cardiomyocytes clearly structured, the cell contains a lot of muscle mass and muscle mitochondria tow large nucleus, nucleolus thick and large, very neat sarcomere. Bundle and mitochondrial closely arranged orderly, muscle tow Z line, A band, M line, H zone and I band visible. Bright and dark bands with very obvious. Bundle beside abundant mitochondria, its densely arranged, mitochondrial membrane integrity, mitochondrial Jing faintly visible inside and long and dense. (Reflecting cellular oxidative metabolic rate) [2) diabetic rat heart cell structure confusion, muscle fiber content was significantly reduced sarcomere less, very irregular, open circuit, was dissolved. Bundle and mitochondrial disordered, muscle tow Z line, A, and M lines with no continuous visible. Light band and dark band disorder, nuclear condensation, nucleolus disappear. Mitochondrial disorganized, few and small, are dissolved, and its membrane is not complete, the steep mitochondrial fusion. (3) diabetic subgroup of myocardial ultrastructure in diabetic rats to be worse than normal. Sarcomere ataxia, muscle bundle and mitochondrial disordered, muscle bundle is split and destroyed. Muscle tow Z line, A band, M line, H zone and I band is not obvious visible. Light band and dark band disorders. Mitochondria into the bundle in a fusiform, mitochondria Jing blurred. However, the extent of damage to light than the diabetic rats. (4) diabetic rats after treatment with rosiglitazone significantly better than no treatment in diabetic rat cardiac structure should be intact, myocardial cell structure clearly still neatly arranged in fascicles. Cells, muscle mass and muscle bundles containing mitochondria, large nuclei, prominent nucleoli exist, muscle tow Z line, A band and M band is also visible. Bright and dark bands with a slightly uneven. Compared with normal cardiac muscle bundle slightly thicker, longer, mitochondria in muscle bundles inserted. Mitochondrial membrane integrity, within the Zhejiang University master's degree thesis Jing faintly visible and long and dense. (Reflecting cellular oxidative metabolic rate is still high) Conclusions: (1) after treatment with rosiglitazone did not make streptavidin streptozotocin (STz)-induced diabetic rats reduced blood glucose, insulin deficiency that its non-diabetic rats hypoglycemic effect. (2) diabetic rat cardiomyocytes structure confusion, muscle fiber content was significantly reduced. Bundle and mitochondrial arranged?
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