Dissertation > Excellent graduate degree dissertation topics show

A Novel Fluorescent Quantitative PCR Detecting Method of Ureaplasma Urealyticum Using Duplex Scorpion Probe

Author: BiZuo
Tutor: YinYiBing
School: Chongqing Medical University
Course: Clinical Laboratory Science
Keywords: Ureaplasma Urealyticum Fluorescence quantitative polymerase chain reaction(FQ-PCR) Duplex scorpion probe
CLC: R450
Type: Master's thesis
Year: 2007
Downloads: 27
Quote: 0
Read: Download Dissertation

Abstract


Objective:To establish a novel real-time fluorescence PCR method to detect ureaplasma urealyticum(UU) with high sensitivity, specificity and stability using the duplex scorpion probe.Method:The recombinant vector pMD18-T-UU-Ure was used as the standard template. The duplex scorpion probe was designed according to the cloned gene sequence. And then the PCR reaction system was optimized and evaluatedResults: 1) The recombinant vector was cloned successfully; 2) Methodology analysis showed the duplex scorpion probe detecting system was established with large detecting range, high sensitivity, specificity, stability and accuracy. 3) After applied in clinical experiments, this novel fluorescent quantitative method has more advantages than the DA’AN UU detecting kit.Conclusion: A novel real time fluorescence quantitative PCR detecting method for ureaplasma urealyticum has been developed, which would lay a substantial foundation for exploring new clinical diagnostic kits using the duplex scorpion probe.

Related Dissertations

  1. Investigation of Infection and Role of Mycoplasma in Infertility,R711.6
  2. The Clinical Research in Children with Refractory Mycoplasma Pneumoniae Pneumonia,R725.6
  3. The Experimental Study on Coptidis Rhicoptidis Total Alkaloids Efferescent Tablet Treating Ureaplasma Urealyticum Infection of Feamle GENITALi,R271.9
  4. Contraceptive and Anti-inflammatory Effects of Jieze No.1,R285.5
  5. Study on the Pathogenicity of Ureaplasma Urealyticum Serotype 3 with Different Concentration in Murine Model,R375
  6. Expression of Bcl-2 mRNA in Reting and Tectum of Zebrafish during Optic Nerve Regeneration,Q3
  7. The Analysis of Autoimmune Antibody Detect Condition about 356 Female Barren Patients,R711.6
  8. Study on Detection, Biovars, Inhibition Test and TetM of Ureaplasma Urealyticum,R518.9
  9. Kangyue Ning treatment of female urinary and reproductive tract Ureaplasma urealyticum infection clinical observation and research,R271.9
  10. Experimental Study on Anti-ureaplasma Urealyticum and Human Cytomegalovirus Effect of TCM Rhynchosia Volubilis Lour and Its Extractives,R285.5
  11. Molecular Epidemiology Investigation of Ureaplasma Urealyticum in Urogenital Tract from Healthy Infants,R181.3
  12. On Study of Relations of Ureaplasma Urealyticum, Chlamydia Trachomatis and Male Infertility,R698.2
  13. 162 cases of adverse pregnancy female reproductive tract Ureaplasma urealyticum distribution of biological research group,R714.2
  14. Study of Relationship between Nongonococcal Urethritis and Herpes Simplex Virus and Curative Effect of Antivirus Therapy,R759
  15. Experimental Study of Zhi-Bai-Di-Huang Decotion in Treating-Ureaplasma Urealyticum and Enhancing Spermatozoa Motility,R285
  16. The Clinical Investigation of Zhibai Dihuang Wan Treating Ureaplasma Urealyticum Prostatitis Complicated with Low Sperm Activity,R277.52
  17. Study of the Clinical Significance of HCMV in Children with Viral Myocarditis,R725.4
  18. Application of Real-Time FQ-PCR in Diagnosis for Ureaplasma Urealyticum,R446.5
  19. Fei Lin Ning Tang treatment of chronic ureaplasma mycoplasma prostatitis clinical observation,R277.52
  20. Antibacterial Activity of Chinese Medicinal Herbs Against Ureaplasma Urealyticum in Vitro,R275.9
  21. The Experimental Study about Inhibitory Effects of Xiao Kang Ling Ⅱ on Uu in Vitro,R285.5

CLC: > Medicine, health > Clinical > Therapy
© 2012 www.DissertationTopic.Net  Mobile