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Firstly, in order to study in HepG2 cells was studied ONOO?, And β-mercaptoethanol on cell SelS, glycogen synthase (glgA), glycogen phosphorylase (glgP) mRNA levels of impact and the role of selenium, and then use alloxan-induced diabetic Wistar rats caused models to study SelS, glgA, glgP mRNA level changes, in order to reveal the selenium and SelS role in the development of diabetes provide evidence. Main results are as follows: 1) low concentration of Na2SeO3, β-mercaptoethanol, ONOO? And SIN-1 on HepG2 cell viability is not significantly affected; higher concentrations of Na2SeO3, β-mercaptoethanol, ONOO? And SIN-1 significantly inhibited cell vitality. Higher concentrations of β-mercaptoethanol treated cell DNA breakage, showing the features of apoptosis, and the amount of the presence of selenium β-mercapto-ethanol-induced apoptosis was significantly inhibited. Low concentrations of Na2SeO3, β-mercaptoethanol, ONOO? And SIN-1 to SelS mRNA levels were significantly increased; while high concentrations of Na2SeO3, β-mercaptoethanol, ONOO? And SIN-1 significantly inhibited SelS mRNA levels; addition, The presence of an appropriate amount of selenium β-mercaptoethanol, ONOO? and SIN-1 SelS mRNA induced a significant increase in the level of effect. Real-time quantitative RT-PCR confirmed the SIN-1 对 SelS mRNA levels. 2) selenium and β-mercaptoethanol on HepG2 hepatoma cells glgA, glgP mRNA levels were not significantly affected; while ONOO?, And SIN-1 is highly regulate hepatic glucose metabolic enzymes glgA and glgP mRNA levels; same time, the appropriate dose of selenium can significantly lower concentrations of SIN-1 on induced glgA, glgP mRNA levels. With the occurrence of apoptosis, high doses of selenium, ONOO?, And β-mercaptoethanol can make HepG2 cells were glgA, glgP mRNA levels declined sharply. 3) application of three consecutive alloxan diabetic rats by intraperitoneal injection method for modeling. The results show that successful modeling of blood glucose rises to 16.7 mM or more, and showed significant weight loss and polyuria symptoms modeling success rate of 60%. Meanwhile, the diabetic rats liver SelS, glgP mRNA levels were significantly increased compared with normal mice, glgA mRNA levels than normal mice was significantly reduced; diabetic rat adipose tissue and skeletal muscle SelS mRNA levels compared with normal mice was not significantly differences in the pancreas SelS mRNA levels than normal mice there is an increase of 2.5 times. Diabetic rat liver lipid peroxidation compared to normal mice significantly increased.
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