Dissertation > Excellent graduate degree dissertation topics show

Ionic Liquids and Carbon Nanotubes in the On-Line Separation and Preconcentration of Proteins

Author: DuZhuo
Tutor: WangJianHua
School: Northeastern University
Course: Analytical Chemistry
Keywords: Ionic liquids Carbon nanotubes Protein Line preconcentration and separation Biological samples
CLC: TB383.1
Type: PhD thesis
Year: 2010
Downloads: 59
Quote: 0
Read: Download Dissertation

Abstract


Ionic liquids and carbon nanotubes as the carrier, to study the performance of the separation and enrichment of proteins in complex matrix samples and extraction mechanism. The specific study includes the following aspects: The first chapter briefly describes the nature of the ionic liquid and carbon nanotubes and its application in the field of extractive separation. Chapter II as a model protein, bovine serum albumin (BSA), were studied by the water-soluble ionic liquid chlorinated 1 - butyl-3 - methylimidazolium [Bmim] Cl (1-Buty1-3-methylimidazolium chloride) and K2HPO4 The formation of the ionic liquid aqueous two-phase system extraction separation of proteins in biological samples. In the phase separation process, the protein is enriched in the ionic liquid phase, in the vertical two-phase allocation ratio of about 10, the enrichment factor of 5. IR and UV spectra are shown the structure of BSA did not change before and after in the extraction. The extraction mechanism studies have shown that the coexistence of the two-phase electrostatic potential energy differences and salting protein enriched in the ionic liquid phase. Chapter III multi-walled carbon nanotubes using nitric acid oxidation line preconcentration and separation basic proteins, in order the different eluent to achieve the basic proteins separation. When the sample volume was 2.0 mL, hemoglobin and cytochrome c enrichment factor of 11 and 15, respectively, retention efficiency are 100%; each of pH 8.0 phosphate buffer solution (PBS) and 0.5 mol / L NaCl solution as eluent selective elution of hemoglobin and cytochrome c, a recovery rate of 98% and 90%, respectively. SDS-polyacrylamide gel electrophoresis results show that the extraction system can be realized on the effective separation of the blood hemoglobin and egg white lysozyme. Chapter oxide layers of self-assembled multi-wall carbon nanotubes fixed on the silica microspheres, in order to improve the the extraction separation performance of carbon nanotubes filled micro-column. By the selection of suitable coating methods and conditions, as much as possible to increase the amount of coverage of the carbon nanotubes, while reducing non-specific interactions of the silica microspheres and the protein. The Langmuir adsorption model of cytochrome c in the multi-walled carbon nanotubes - polyelectrolyte coated silica particles (MWNTs/SiO2) filled micro-column adsorption behavior was investigated. Optimal experimental conditions, the adsorption capacity of the adsorbent cytochrome c 112 mg / g. When the sample volume of 2.0 mL, the cytochrome c enrichment factor of up to 30. Chapter positively charged water-soluble polyelectrolyte poly dimethyl diallyl ammonium chloride (PDDA) non-covalent modification of carbon nanotubes, in order to change the nature of the charge of the carbon nanotube surface and rendered hydrophilic , in order to achieve the adsorption of acidic protein, to avoid the destruction of the chemical modification method of a carbon nanotube structure to exclude the introduction of the group having a non-specific adsorption of protein. Optimal experimental conditions, when the sample volume and elution volume of 2.0 mL and 200μL, bovine serum albumin retention efficiency and elution efficiency were 100% and 90%, the enrichment factor of 14. Chapter VI polyelectrolyte functionalized carbon nanotubes ultrasonic dispersion on the basis of these studies, in the microporous film of 1.2μm pore size mixed fibers forming a thin layer of a thickness of about 5μm the carbon nanotube. The carbon nanotube composite membrane into the the online film extraction module, combined with sequential injection system online membrane extraction of acidic proteins (such as bovine serum albumin), retention of the protein can be a citrate buffer solution effectively quantitative elution. The membrane extraction system significantly improve the adsorption capacity of bovine serum albumin (BSA), or 3.8 mg / mg (1.4 mg/cm2), 146 times micro-packed column extraction methods.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Recovery Whey Soy Protein by Microfiltration and Development of Whey Powder,TQ936.2
  3. Research on Disease Related miRNA Mining Based on Protein-Protein Interaction Network,R341
  4. The Research and Implementation of Protein Classification Algorithm on the Basic of String Kernel,TP301.6
  5. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  6. Expression of hBMP4 and hBMP7 in Chinese Hamster Ovary Cells,Q78
  7. The Study of Color Change and Control Technology for Oyster Protein Beverage,TS254.4
  8. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  9. Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus,R587.1
  10. Impact on Drosophila Brain Developement Through the Overexpression and Loss of Function of Gsα,Q75
  11. Study on Deodorization Techniques of Oyster Protein Beverage,TS254.4
  12. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  13. Synthesis of Benzimidazolium Ionic Liquids and Their Application in Extraction Separation,O626.23
  14. Study on Preparation and Properties of Protein Isolates from Tilapia,TS254.4
  15. Expression and Purification of Influenza a Virus M2 Protein and Detection of Its Antigenicity,R392
  16. Broiler Responses to Reduced-protein Diets Suppleme Nted with Synthetic Amino Acids and Small Peptides,S831.5
  17. The Preparation of Hpv58e7 Protein by GST-tagged Prokaryotic Expression System,R392
  18. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  19. Comparative Study on Reproductive Biological Characteristics of Helicoverpa Armigera and Helicoverpa Assulta (Lepidoptera:Nuctuidae),S433
  20. Effects of Low-protein Diet on Production Performance and Feeding Environment of Piglet,S828.5
  21. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78

CLC: > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials
© 2012 www.DissertationTopic.Net  Mobile