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Effect of the Bone Formation and Biocompatiable of Complex Particles Composed by Cerium and Bioglass in Rabbit Femoral Defect

Author: HuangHai
Tutor: WangZhen
School: Fourth Military Medical University
Course: Surgery
Keywords: Bioglass Ceria Biocompatibility Bone formation
CLC: R318.08
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Abstract

Background: The war wounds, severe trauma caused by a car accident, including earthquakes, landslides and other natural disasters as well as osteomyelitis, bone tumors, such as major surgery can cause a large area of ??the bone defect. Now usually selected for such a large area of ??the bone defect clinical the implantable biological material to fill the defect and to promote new bone formation, otherwise it is difficult to ensure that the positive effect of treatment. However, the selection of the repair material so far no satisfactory answer. The usual defect repair materials according to their sources can be divided into three types: autologous bone, allograft bone, artificial bone. Since bone graft has been the gold standard as a bone graft, but more limited sources, it is difficult to widely used clinically. Today, technological advances in biological materials, clinical doctors and medical science and technology workers more and more sights bone. Objective: To study the bioactive glass particles implanted into bone defect early performance degradation. And control ceria composite bioactive glass particles compared to whether the differences in the osteogenic implant defects early performance with simple biological glass particles. At the same time to evaluate the inflammatory response observed ceria composite bioactive glass particles have anti-inflammatory capabilities. Discussion dioxide whether cerium can be used as a new material for the field of tissue engineering and test the new composite material can be integrated two material advantages. Material: a purely biological glass particles: 2 dioxide cerium composite biological glass particles. Methods: 6 Weight 2.5-3.0KG mature New Zealand white rabbits. Divided into two weeks and four weeks, two groups, each group of three rabbits, each rabbit around the femoral condyle manufacturing 0.6CM a cylindrical through injury model, were randomly implanted ceria composite bioactive glass particles and simple type bioactive glass particles, each rabbit were implanted ceria composite bioactive glass particles of bioactive glass particles alone. Vital signs and health status were observed after the experimental animals, and observe the local wound healing state, largely determine the degree of the inflammatory response. Respectively, in the experimental animals were sacrificed by grouping remove specimens after 2 weeks and 4 weeks. Fixed in 10% neutral formalin, dehydrated, hard tissue embedded, sliced, bone formation and degradation do VG staining, light microscopy material implant site. The image analysis data to calculate the area of ??new bone defect area, and the remaining material area. Statistical analysis was performed. Results: The patients all six animals were normal survival to the sampling time, during which vital signs and health status are in the normal range. General observation that the wound healed well, no severe inflammatory response. Light microscope, two weeks when the two materials are planted defects have a bone regeneration, bone formation rate between the two materials freshmen, no statistically significant difference (P = 0.61, P gt; 0.05). The amount of new bone four weeks when the two materials increased significantly (pure bioactive glass particles group P = 0.024, ceria composite bioactive glass particles group P = 0.005, both P values ??less than 0.05). At 4 weeks, compared to bone regeneration rate between the two materials, the difference was not statistically significant (P = 0.62, P gt; 0.05). Two weeks and four weeks when the materials were no significant degradation, the residual amount of material in the two time periods material residual amount of contrast between the two materials contrast there was no significant difference (P gt; 0.05). Conclusion: bioactive glass particles in the two weeks after that can promote new bone formation, new bone generated in the the defect surrounding bone material junction, then gradually spread to the defect center growth, 4 weeks observed in the material center of the newborn bone. Ceria composite bioglass not cause inflammation, and its the osteogenic ability to contrast bioactive glass particles alone was no difference, good biocompatibility, new materials to conduct a further study can be used as a tissue engineering.

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