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Objective To observe the diabetic rat liver fatty liver morphological changes, oxidative stress and TRx expression of probucol on diabetic fatty liver treatment, and to explore the possible mechanism of action. Methods 40 male Wistar rats were fed for a week, were randomly divided into normal control group (10) and experimental group (30). Normal control group were fed with normal diet, the experimental group were fed with high-fat high-sugar diet, 4w after the experimental group received 35mg/kg body weight dose intraperitoneal injection of STZ solution was to create a model of diabetes, high sugar, high fat diet continue 4w after death experiment rats 3, confirming diabetic fatty liver rat model successfully, then the successful modeling of 22 rats were divided into two experimental groups: model group (11), probucol intervention group (11), probucol group received probucol 500mg / (d · Kg) orally, the normal control group and model group were given an equal volume of 0.5% CMC solution volume gavage. After 8 weeks all animals were sacrificed each group were compared fasting blood glucose (FBG), liver weight, body weight, liver index, blood lipids, liver function, liver lipids, superoxide dismutase (SOD), malondialdehyde ( MDA), thioredoxin (TRx) and liver histopathology. Results 1 diabetic model, based on, and then continue to be high-sugar high-fat diet for 4 weeks, the rats had increased liver, liver lipid deposition, hepatic steatosis and inflammatory cell infiltration characteristic of fatty liver pathological changes. 2, to 17 weekends rats, FBG, water intake, ALT, AST, serum TG, serum TC, LDL, liver TG, liver TC, liver weight, liver index, MDA, blood TRx content, liver TRx expression, liver fatty degeneration and inflammatory cell infiltration scores were significantly higher than the control group (all p lt; 0.01). Blood HDL, SOD, SOD / MDA ratio was significantly lower than the control group (all p lt; 0.01). 3 to 17 weekend, probucol group liver weight, liver index, ALT, AST, liver TC, blood TC, LDL, MDA, serum TRx, TRx expression of liver, liver steatosis and inflammation scores were significantly lower than model group (all p lt; 0.01), its water consumption is also significantly lower than the model group (p lt; 0.05). Probucol rats body weight, SOD, SOD / MDA was significantly higher than the model group (p lt; 0.05). The intervention group probucol FBG, liver TG, TG and HDL blood compared with the model group had no significant difference. 4, each rat serum and serum SOD TRx content was negatively correlated, Pearson correlation coefficient (γ = -0.954, p lt; 0.001), serum and serum levels of MDA TRx positive correlation, Pearson correlation coefficient (γ = 0.820 , p lt; 0.001). Conclusion 1 diabetic rat liver fatty liver abnormal liver function, increased liver, liver lipid deposition, hepatic steatosis and inflammatory cell infiltration and other pathological changes, after 8 weeks of probucol treatment can reduce its pathological conditions, suggesting Probucol fatty liver of diabetic rats have a therapeutic effect. 2, MDA probucol group was significantly lower than the model group, while SOD and SOD / MDA was significantly higher than the model group, suggesting that probucol fatty liver of diabetic rats and improvement of the therapeutic effect of oxidative stress related. 3, the model TRx serum levels were significantly higher than the control group, while the probucol group was significantly lower than the model group, and the serum levels of serum SOD TRx was negatively correlated with serum MDA levels were positively correlated, suggesting that TRx levels and diabetic fatty liver of oxidative stress is consistent. 4, liver TRx model group was significantly higher than the normal control group, while the probucol group than in model group, suggesting that diabetes, fatty liver oxidative stress in rat liver exist, and probucol may improve its oxidative stress.
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