Dissertation > Excellent graduate degree dissertation topics show

The tumor resistance new target receptor structure - based lead compound discovery and research

Author: ZhouYuan
Tutor: YangChunZheng
School: Peking Union Medical College , China
Course: Molecular Pharmacology
Keywords: Target Sorcin Multidurg resistance Integrinαvβ3 Virtual screening Lead compound QSAR Drug design
CLC: R91
Type: PhD thesis
Year: 2005
Downloads: 243
Quote: 0
Read: Download Dissertation

Abstract


In the post-genomic era, researchers are faced with the enormous challenge of translating genomic information into new innovative medicines to improve the human condition. Thousands or more potential new drug targets have emerged as a result of sequencing the human genome, therefore, genomic data can then be used to focus structure-based inhibitor design towards areas of the novel target molecules. If we are to effectively use the fruits of genomic research, we must re-engineer drug discovery and development. There are two key processes in drug discovery today, the identification and validation of new drug targets and the identification and optimization of leads from them.1. Validation of Sorcin, an Important Gene Associated with Multidrug -Resistance in Human Leukemia Cells.Multidrug resistance (MDR) in cancer, especially in leukemia, represents a major obstacle to successful chemotherapies. The mechanisms of MDR are polygenetic and yet to be completely defined. By gene profiling analysis we previously identified 12 genes that showed significantly altered signal density in a doxorubicin-induced MDR leukemia cell line, K562/A02, compared with its parent cells K562. One of the genes significantly up-regulated in K562/A02 cells is sorcin (soluble resistance-related calcium-binding protein). We have also demonstrated that the level of sorcin expression in leukemia patients correlates not only directly with that of the mdr 1 gene, but also inversely with patients’ response to chemotherapies and overall prognosis.In this study we have carried out experiments to dissect out the contribution of sorcin by itself to drug resistant phenotype in K562 cells. The full-length sorcin gene was amplified by RT-PCR from K562/A02 leukemia cells, and cloned into pcDNA3.1 eukaryotic expression vector. After transfection of K562 cells with the pcDNA3.1/sorcin and subsequent G418 selection, individual clones were isolated

Related Dissertations

  1. The Key Technologies of Autocollimation System Based on the Beam-Splitting Target Detecting Feedback of Laser Beam Shift,TH741.14
  2. Maneuver Detection and Tracking with the Radar Rate Measurement,TN953
  3. The Maneuvering Target Tracking Research Based on VRPF,TN957.52
  4. ISAR Imaging Simulation of Space Targets and Target Recognition Based on ISAR Images,TN957.52
  5. Research on Multi-Sensor Embattling and Continious Tracking Technique Applying for Anti-Stealth and Anti-Interference,TN953
  6. The Research and Implementation of Software Oriented Fault Injection Tool,TP311.52
  7. Research on Joint Target Detection for Dual-Sensor Image and System Implementation,TP391.41
  8. Study on Virtual Detector of Infrared Hyper-Spectral Image,TP391.41
  9. Research on the Image Real-Time Acquisition, Storage and Image Processing System,TP391.41
  10. Study on Nonlinear Variable Structure Guidance Law,TJ765
  11. The Research of Guidance and Control Method in Terminal Guidance Phase,TJ765.3
  12. Research on Target Tracking and System in Wireless Sensor Networks Based on Character of Movement and Terrain Restriction,TP212.9
  13. Application and Research of Dual Control in Vertical Landing Aircraft,TP273
  14. Comprehensive Quality Assessment of College Students,G645.5
  15. Marketing Strategy of China campus buy,F274
  16. Computational Identification and Characterization of Microrna Genes from Agricultural Insects,S186
  17. Identification and Analysis of miRNA and Flower Development Specific Genes in Wheat,S512.1
  18. Multi-target Radiofrequency Lesion for Patients with Intractable Psychiatric Disorders,R749
  19. Research for Infrared Image Target Identification and Tracking Technology,TP391.41
  20. Space Infrared Target Simulation and Application of Target Trace,TP391.41
  21. The Compression and Fusion Technique Research of Underwater Target Feature,TN911.7

CLC: > Medicine, health > Pharmacy > Drug basic science
© 2012 www.DissertationTopic.Net  Mobile