Dissertation > Excellent graduate degree dissertation topics show

Study of Application of Ionic Liquid in Sample Pretreatment and Chemiluminescence in Chromatographic Analysis

Author: WuHongWei
Tutor: ZhuYan; ChenMeiLan
School: Zhejiang University
Course: Analytical Chemistry
Keywords: Ionic liquid (IL) sample pretreatment high performance liquidchromatography (HPLC) ion-exchange chromatography (IC) chemiluminescence(CL)
CLC: O657.7
Type: PhD thesis
Year: 2012
Downloads: 275
Quote: 0
Read: Download Dissertation

Abstract


In recent years, because of the complex matrix and low analyte content of sample of HPLC analysis, it is very important to develop new types of sample pretreatment methods and sensitive detection methods.In this work, the application of ionic liquid-based extraction techniques in sample pretreatment of different chromatographic methods was investigated. The combination of ion chromatography and chemiluminescence was also studied.Chapter one comprised a brief introduction of ionic liquid and its application in analytical chemistry and a comprehensive review of the application of ionic liquid extraction methods in sample pretreatment of chromatography. The information of chemiluminescence such as principle, apparatus structure, typical chemical luminescence system and combination of HPLC were summarized.In chapter two, a novel ionic liquid-based pressurized liquid extraction (IL-PLE) procedure coupled to high performance liquid chromatography (HPLC) tandem chemiluminescence (CL) detection capable of quantifying trace amounts of rutin and quercetin in four Chinese medicine plants was described. The effect of ILs with different cation on extraction efficiency was systematically investigated. In addition, the extraction performance of IL-PLE was compared with HRE and UAE for the extraction of rutin and quercetin in real samples. What is more, the selectivity of the proposed method was investigated by comparing the chromatograms of real sample obtained with CL detector and UV detector.In chapter three, the ionic liquid-based microwave-assisted dispersive microextraction (IL-based MADME) was firstly used to solid sample. The aim of this work is to simplify the analytical step, reduce the extraction time and improve the sensitivity. The effects of various experimental parameters were studied and the possible extraction mechanism was also discussed. The developed method was successfully applied to the analysis of real samples.In chapter four, a novel method was established for the simultaneous determination of resorcinol and phloroglucinol in environmental water samples by ion chromatography with chemiluminescence detection. The absence of methanol and acetonitrile in mobile phase made the proposed method more sensitive.In chapter five, without complicated derivative process and gradient elution, a simple, fast, sensitive, highly selective and eco-friendly analytical method for the determination of catecholamines in human urine was established originally in this paper, based on the combination technique of IC with the CL detection. Parameters related to IC separation and acidic potassium permanganate chemiluminescence system were optimized systematically.

Related Dissertations

  1. Study on Sample Pretreatment Techniques in Single Photon Ionization-Time of Flight Mass Spectroscopic Analysis of Volatile Organic Compounds,O657.63
  2. Analysis of Benzene Homologues, Preservatives and Hormones by Chromatography,TS207.3
  3. Research and Application of New Sample Processing Methods in Pharmaceutical Analysis,R917
  4. Study on Electrodialysis Pretreatment Technique Used for Sample Neutralization, Oxidation, Extraction and Its Application in Ion Chromatography,O657.75
  5. Study on the Novel Sample Pretreatment Methods in Ion Chromatography,O657.75
  6. Study on Effects of Sampling Disturbance in Port Prospecting at Hainan Province,U652.2
  7. Study on Opening AAS Virtual Laboratory Construction,O657.31
  8. Study and Application on Analysis Techniques of Pesticides Residues in Foodstuff,X83
  9. Determination of Drugs in Biological Fluid by Liquid-phase Microextraction Combined with High-performance Liquid Chromatography,O657.72
  10. Study on Capillary Electrophoresis with Dialysis Sampling Function,O658.9
  11. Trace and ultra- trace dioxins high resolution gas chromatography / high resolution mass spectrometry analysis technique,X132
  12. Determination of Seven Sulfonamides Residues in Poark by Liquid Chromatography-Mass Spectrometry,TS251
  13. Environment triazine pesticide residues testing and evaluation,X592
  14. Application of Ionic Liquids in Sample Pretreatment of Chromatography,O657.75
  15. Green Analytical Methodologies in Liquid-Phase Microcextraction Combined with Capillary Electrophoresis,O652.6
  16. Study on Biological Sample Pretreatment Chip System,TN492
  17. Sample Pretreatment and Chromatographic Analysis in Determination of Toxic Residues in Food,TS207.3
  18. Technology and Method Development for Sample Pretreatment and Automated Analysis in Proteome Research,O629.73
  19. Two Dimensional Pre-Separation System Based on Short Carbon Fibers Column for Hplc-UV Analysis of Plant Sample,O657.72
  20. The Preparation and Application of the Poly (vinyl ester resin) Organic-inorganic Hybrid Capillary Monolithic Columns,O657.72

CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Chromatographic analysis
© 2012 www.DissertationTopic.Net  Mobile