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Study on Anti-radiation Effect of KGF and G-CSF on Mice Irradiated with5.0Gy γ Ray

Author: YuChengCheng
Tutor: JiangEnHai
School: Beijing Union Medical College
Course: Radiation Medicine
Keywords: Keratinocyte growth factor Granulocyte colony stimulating factor Hematopoieticsystem Immune system Bone marrow hematopoietic suppression Oxidative damage
CLC: R818.74
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
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Abstract


ObjectiveTo investigate the protective effect of KGF+G-CSF on mice irradiated by5Gy gamma ray and then do some research on the mechanism. The purpose of our research is to provide an important theoretical basis for cytokine combination therapy on acute radiation sickness.MethodsC57BL/6mice were selected to receive whole body irradiation of137Cs γ ray to build a mouse model of acute radiation sickness. Test the effects on the blood and immune system in irradiated mice by the indexes of body weight, organ indexes, white blood cells, red blood cells, concentration of hemoglobin and blood pelets in peripheral blood, colony forming units of spleen and so on. Test the effects on bone marrow hematopoietic system in irradiated mice by the indexes of bone marrow nucleated cells, bone marrow DNA contents, listeria-granulocyte colony forming unit (CFU-GM), red blood cell blowout type colony generating unit (BFU-E) and so on. Detect the activity of superoxide dismutase (SOD) and content of Maleic Dialdehyde (MDA) to reflect its effect on antioxidation in irradiated mice.ResultsThe weight of combined drug group is significantly recovers better than other groups. Compared with simple IR group, combined drug group can reduce the damage to the peripheral blood caused by radiation. Joint drug delivery can improve thymus coefficient, spleen coefficient, the number of CFU-S irradiation mice, and protect the immune system in radiated mice. Joint drug delivery can ameliorate BMNC, bone marrow DNA content, CFU-GM, BFU-E caused by radiation obviously, and protect the bone marrow hematopoietic system from radiation damage. Joint drug delivery can increase the SOD activity in liver tissue, decrease the MDA content, and protect the liver tissue caused from radiation damage. ConclusionsDifferent drug groups are of different protective effects on hematopoietic system, immunity system and the organization organs of irradiation mice. This study indicates that KGF in combination with G-CSF is of some protective effect from radiation damage on C57BL/6mice.

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CLC: > Medicine, health > Of Medical > Radiation Medicine > Radiation sickness,radiation damage > Radiation sickness and radiation injury on the > Radiation injury
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