Dissertation > Excellent graduate degree dissertation topics show

Preliminary Study on Redox State of HypoxicMouse Brain by Cryo-Imaging

Author: ZuoHanBing
Tutor: ZhangZhiHong
School: Huazhong University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Redox state Low temperature environment of liquid nitrogen Intrinsicfluorescent molecular Hypoxic mouse brain Cryo-imaging
CLC: R318.52
Type: Master's thesis
Year: 2012
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


That, the brain tissue redox metabolic continues well, is the premise to maintain normalbrain structure and function. It needs continuous and effective blood supply to keep thebalance of brain tissue metabolic redox status, because even a subtle change of blood flowor blood oxygen may lead to dramatic change of the metabolic redox status, which resultsin the concentration change of NADH and Fp in the brain cells’ mitochondria. In this thesis,we have combined the endogenous fluorescent molecular imaging techniques with thelow-temperature fixation technology of tissue energy metabolic status, and access to theinformation of the multi-sectional distribution of the whole Kunming mouse brainmetabolic redox status under hypoxia. The research would be helpful for raising awarenessof the relationship between the brain energy metabolism under different physiological andpathological conditions and the endogenous coenzyme, and supplying methods for thebrain metabolic abnormal specific diagnosis, treatment and following steps.This thesis includes:1. Three common fixation methods, decapitation freezing method, direct freezingmethod and funnel freezing method, were compared. At last, we decided to choose thefunnel freezing method according to the fixed complete degree of the sample structure andthe sample status homogeneity.2. According to the relationship between rate and temperature the of biochemicalreactions inside the body, we knew that to ensure a stable fixation metabolic state, we mustkeep them in liquid nitrogen. Therefore, this thesis explored the low temperaturemaintenance technology using self-designed corresponding modules to keep the sample inliquid nitrogen condition.3. We used the multi-channel three-dimensional fluorescence imaging system toacquire the mouse brain’s NADH and Fp coenzyme concentration distribution informationof death and different physiological conditions under the environment of low temperaturesin liquid nitrogen. Moreover, we found that the hypoxic mouse brain was in overall high oxygen reduction; and the regional difference between brain white matter and gray matterof the hypoxic and normal brains was just the opposite.

Related Dissertations

  1. A Coupled Enzyme Assay for Redox and Activity of Protein Targeted by Maize Chloroplast Thioredoxins,S513
  2. Effect and Mechanism of Antioxidant Factors on Lipid Metabolism in High-fat Diet-fed Mice,R589.2
  3. Effects of Glutathione on Dengue Virus Production in HepG2 Cell,R373
  4. Effect of High Fat Induced-Oxidative Stress on Somatostatin Secretion and Gene Expression of Intestine and Liver,R363
  5. pH-sensitive and Redox-sensitive Fluorescent Probes and Their Application in the Imaging of Tumor Cells,R730.4
  6. The Changes and Clinical Significance of Glutathione Redox-state in Patients with Coronary Heart Disease,R541.4
  7. Changes of Redox State in Host Cells during Dengue Virus Infection,R373
  8. Effects of Rare Earth Elements on Isoflavone Biosynthesis and Redox Status in Cells Suspension Cultures of Maackia Amurensis,S567.19
  9. Studies on Signal Regulation of Programmed Cell Death Induced by NaCl in Vicia Faba L. Guard Cells and Epidermal Cells,Q942.5
  10. The Alterations of Redox Status、Antioxidative Capability of Malignant Tumors and Its Association with Apoptosis,R730.2
  11. Effect of K562 Cell Conditioned Medium on Redox State in Endothelial Cells and Its Significance on Tumor Angiogenesis,R730.2
  12. Study on the Correlation between Plasma GSH/GSSG、Serum NADPH/NADP~+ and OSAHS、OSAHS Associated Hypertension,R544.1
  13. Regulatory Study on Molecular Mechanism of State Transition in Cyanobacteria,Q946
  14. Red and gold colored glass redox state of the impact of its,TQ171.1
  15. The Initial Explore of Relationship with Redox and Status Yin-yang,R226
  16. The Effects of Hydrogen Perxide on the Redox State of Human Dental Pulp Cells,R783
  17. Exogenous Antioxidants on broiler body redox status and production performance,S831
  18. Study on the Redox Regulation of Somatostatin in Diabetes Mice Induced by STZ,R587.1
  19. Effects of High Ambient Temperature and High Humidity on Redox State and Immune Function in Growing Pigs,S828
  20. Effects of Somatostatin Analogue Octreotide on Redox Status of Mice Fed with High-fat,R965
  21. Effects of Oxidized Soybean Protein on the Redox State and Liver Gene Expression in Mice,R151

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Other branches of biomedical engineering > Low temperature biomedical
© 2012 www.DissertationTopic.Net  Mobile