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The background since bone integration (osseointegration) theory became the dominant theory of Oral Implantology development a wide range of development and application of Oral Implantology. However, Implant Restoration longer healing time after implantation, bone, poor bone parts high failure rate. Implant - tissue interface reaction is considered to determine the dental implant success and failure of one of the important factors, scholars have effective control by the surface of the planting material to promote bone integration. In recent years, on the roughened implant surface, and activation of the surface oxide basis, the biologically active molecule complex biochemical modification of the implant surface to become more active and fast-growing area of ??the implant design study. Commonly used active ingredient in the matrix of the titanium surface modified in order to simulate the extracellular matrix of the regulatory functions of the biological behavior and to obtain the degree of certainty of results. But these single active ingredient with natural substrates are still gaps. Therefore, in order to bionic construct closer to the natural situation, the present study Imagine with the biological self-secretion of the extracellular matrix, as a new type of biological material, the preliminary study of the titanium implant. The purpose of the titanium surface modification on the biological behavior of the bone marrow stromal cells in vitro studies into bone cells secreted extracellular matrix (ECM), and provide the basis for further guidance titanium implant surface bionic build. Methods SD rats, to modified tissue cultured osteoblasts. Take 12 mm diameter titanium rod, processed into a 1 mm thick titanium sheet, after disinfection into 24-well culture plate, and DMEM culture medium which will become a bone cell concentration was adjusted to 3 × 105/ml, take 1 mL / well seeded in titanium plate left off after repeated freezing and thawing cells matrix, is the matrix of titanium sheet (Ti / ECM). SD rats, cultured bone marrow stromal cells (BMSCs) whole blood adherent method, a spread to the 3rd generation of cells with DMEM medium concentration was adjusted to 1 × 105/mL take 1 mL / holes were inoculated divided into a matrix of titanium the matrix titanium plate and titanium sheet, piece group (Ti / ECM / BMSCs), titanium plate group (CpTi / BMSCs). By immunofluorescence, scanning electron microscopy observation and MTT assay, ALP activity detection observe the change of the surface morphology of the matrix of titanium sheet, extracellular matrix morphology and identify the ingredients and compared between the two groups on the titanium plate early adhesion of bone marrow stromal cells. spread, growth and proliferation, and differentiation. Results on titanium plate matrix of build, fluorescence microscopy showed acellular titanium surface there are biologically active components of the extracellular matrix, rendered as irregular flocculent, SEM shows the matrix after the titanium surface is a layer-like jelly covering material; compared two groups of cell behavior, SEM show that bone marrow stromal cells in the matrix of titanium surface adhesion, spreading good vaccination 4h, Ti / ECM / BMSCs cell adhesion CpTi / BMSCs group, a significant difference (P lt; 0.05), after inoculation 1,3,5,7 days, cell proliferation and CpTi between groups of the Ti / ECM group there were significant differences (P lt; 0.05); 5 days after the inoculation, Ti / ECM, the surface of the cell differentiation with CpTi between the existence of a significant difference (P lt; 0.05). Conclusion After the initial matrix of construction, bio-active ingredients of titanium surface there are a variety of extracellular matrix, the matrix of titanium piece is more conducive to bone marrow the early stromal cells adhesion and further spreading, proliferation, and may have induced the cells to osteoblasts differentiation.
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