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In this paper, the free culture method to study the soil Xinjiang hot springs prokaryotic microbial diversity and biogeographic. By constructing bacterial and archaeal 16S rDNA clone library analysis of the hot springs, Manas soil bacteria and archaea diversity. T-RFLP method, Manas three Shihezi a hot air springs soil sulfur oxidation and sulfur cycle bacteria diversity and environmental factors, the relationship between the location of the sample point. The main contents and results are as follows: from the hot springs, Manas soil bacterial 16S rDNA clone library randomly selected 170 positive clones with Hae III restriction enzyme RFLP analysis, by typing sequencing, build The phylogenetic tree, get 29 different taxa (operational taxonomic unit, OTU). Phylogenetic analysis grouped into six doors: acid bacillus door (Acidobacteria), Actinobacteria (Actinobacteria), Bacteroides door (Bacteroidetes), the Firmicutes (Firmicutes), the Proteobacteria door (Proteobacteria) and floating mildew doors ( Planctomycetes), Firmicutes (Firmicutes) absolute advantage in groups, accounting for 71% of the entire bacterial library. The 29 OTUs There 14 sequences in GenBank sequence similarity less than 97% (sequence length of approximately 1.5 kb), accounting for 48% of the total number of sequences. The results showed that the hot spring soil bacterial diversity is lower, but there are a large number of potential new bacterial species. 16S rDNA clone library Manas hot springs soil archaea randomly selected 98 positive Takako Hha Ⅰ restriction enzyme RFLP analysis, by typing sequencing, phylogenetic tree was constructed, and a total of 16 different taxa (operational taxonomic unit, OTU). Phylogenetic analysis are the property the Crenarchaeota door (Crenarchaeota), part of the door two taxa (Group Ⅰ and Group II). The results show that the dominant habitat in the hot springs soil Archaea Crenarchaeota. 3 from the Manas three hot springs and Shihezi City, a hot springs in the total soil DNA amplification sulfur oxidation and sulfur reducing bacteria arp A gene T-RFLP analysis, combined determination of soil environmental factors parameters by polyethylene class analysis, principal component analysis (PCA) and canonical correlation analysis (CCA), the results showed that the soil temperature and pH affect hot springs the soil sulfur oxidation and sulfur-reducing bacteria diversity, that is, within a certain range, soil temperature and pH can predict The the soil sulfur oxide and sulfur-reducing bacteria Diversity, and other environmental factors. Manas three hot springs and Shihezi City a hot springs in the total soil DNA amplification of 16S rDNA T-RFLP analysis, and combined with the determination of the parameters of soil environmental factors, cluster analysis, principal component analysis (PCA) and canonical correlation analysis (CCA), the results show that soil organic matter content in addition to temperature and pH, ammonium nitrogen and nitrate content of physical and chemical factors affecting soil bacterial diversity, in addition to location differences between the samples also affect soil bacteria Diversity.
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