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Objective To study the protective effect of the supplement Bifidobacterium to reduce intestinal tissue PAF and improve intestinal tissue SIgA, to provide a scientific basis for the application Bifidobacterium prevention and treatment of neonatal necrotizing enterocolitis. Methods 84 two-day-old newborn SD rats, male or female, were randomly divided into three groups: the experimental group (A), the control group (B), blank control group (C), each group n = 28. A: Each 0.04g (including 200 million viable cells) Bifidobacterium gavage (0.2ml / with PH7.4 saline preparation), continuing a week, once a day; B and Group C: each 0.2 ml normal saline week, once a day. A week after the experimental group and the control group were injected LPS (5mg/kg, paired with saline 5mg/ml) necrotizing enterocolitis model. Three groups after intraperitoneal injection of LPS in group A and group B, 2, 6, 12, and 24 hours for each time point were randomly selected 7 with neck breaking, were killed, dissected the bottom of the specimens from the duodenum to the rectum, the upper end of intestinal tissue , the ileocecal proximal intestine to observe the the SD rats pathological specimens, and in the remaining the intestinal the ELISA detection intestinal tissue homogenates PAF SIgA content. SPSS16.0 statistical software, the result of the measurement data expressed as mean ± standard deviation (ˉ X ± SD), the groups were compared using the t-test, P lt; 0.05 prompt the difference was statistically significant. Results. Various experimental groups of neonatal SD rat intestinal tissue pathomorphology newborn SD rats were injected with LPS group B neonatal SD rats were lazy move, malaise, 1-2 hours to appear bloating, diarrhea 6-12 hours to the above symptoms, abdominal breathing faster, lips cyanosis, lack of exercise, body hair messy, less shiny, severe convulsions, cyanosis of the limbs, whole body cool, even death; animals were sacrificed, see gastric retention, bowel wall and mesenteric congestion, increased intestinal dilatation, edema, intestine exudate, see serious intestinal wall point, flaky hemorrhage and necrosis, the symptoms of the 24-hour survivors. HE staining under light microscope changes: neonatal SD rats after LPS injection, group B 2 hours of intestinal epithelial cell degeneration, villous atrophy; six hours the visible intestinal epithelial structure completely disappeared, fluff glands off, missing, lamina propria capillary congestion, inflammatory cell infiltration; 12 hours intestinal villus epithelial cells to begin the repair, but unclear villous architecture; 24 hours after the structure of the intestinal villi. Group A than in group B compared with mild symptoms. 2 groups of neonatal SD rat intestinal tissue PAF of SIgA in content change the content of the B group of intestinal tissue PAF 2h, 6h, 12h and C group had significantly increased (P lt; 0.05) A group of intestinal tissue PAF content 2h, 6h, 12h compared with group B was significantly reduced (P lt; 0.05). A group of intestinal tissue SIgA of content in 2h, 6h, 12h and C group comparison there is a significant increase (P lt; 0.05), group B intestinal tissue SIgA of content in 2h, 6h, 12h and A group comparison have significantly reduce (P lt ; 0.05). Conclusion 1. Endotoxin-induced necrotic enterocolitis in newborn rats model, exogenous administration of Bifidobacterium intestinal tissue of the experimental group the PAF's content with the control group were significantly lower, indicating that bifidobacteria may PAF in the inflammatory cascade by inhibiting expression protect the intestinal tract, can reduce the model intestinal tissue pathological changes of necrotizing enterocolitis in newborn rats. 2 endotoxin-induced necrotic enterocolitis in newborn rats model of exogenous more obvious increase in the the SIgA content of the control group given Bifidobacterium intestinal tissue of the experimental group, indicating that bifidobacteria may protect the intestinal by intestinal immune SIgA expression and road.
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